Cancer Biomark. 2019;25(2):133-139. doi: 10.3233/CBM-181727.
Long noncoding RNAs (LncRNAs) are involved in the occurrence and progression of human tumors including ovarian cancer (OC). Long noncoding RNA HOTTIP has been found to be involved in several human tumors development. However, the role of HOTTIP in OC remains large unknown. In the present study, our results observed that lncRNA HOTTIP expression levels were notably higher in ovarian cancer tissue samples compared to adjacent normal tissue samples. Increased lncRNA HOTTIP expression levels were significantly associated with advanced FIGO stage and lymph node metastasis of ovarian cancer patients. Survival plots analysis results showed high lncRNA HOTTIP expression levels in ovarian cancer patients showed a poor prognosis compared to patients with low lncRNA HOTTIP expression levels. Function assays showed that lncRNA HOTTIP knockdown in ovarian cancer cells decreased cell proliferation and cell invasion capacities. Furthermore, we demonstrated that inhibition of lncRNA HOTTIP suppressed Wnt/β-catenin signaling by downregulating β-catenin expression. Thus, these results suggest that aberrant HOTTIP expression level could serve as a promising biomarker for monitoring ovarian cancer and potential target of ovarian cancer treatment.
长链非编码 RNA（lncRNAs）参与了包括卵巢癌（OC）在内的人类肿瘤的发生和发展。长链非编码 RNA HOTTIP 已被发现参与了几种人类肿瘤的发展。然而，HOTTIP 在 OC 中的作用仍知之甚少。在本研究中，我们的结果观察到，lncRNA HOTTIP 的表达水平在卵巢癌组织样本中明显高于相邻的正常组织样本。lncRNA HOTTIP 表达水平的增加与卵巢癌患者FIGO 晚期和淋巴结转移显著相关。生存曲线分析结果表明，与低表达 lncRNA HOTTIP 的卵巢癌患者相比，高表达 lncRNA HOTTIP 的患者预后较差。功能测定表明，卵巢癌细胞中 lncRNA HOTTIP 的敲低降低了细胞的增殖和侵袭能力。此外，我们还证明，通过下调β-catenin 的表达，抑制 lncRNA HOTTIP 抑制了 Wnt/β-catenin 信号通路。因此，这些结果表明，异常的 HOTTIP 表达水平可以作为监测卵巢癌的有前途的生物标志物和卵巢癌治疗的潜在靶点。
