Immunology Microbiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, 799102 Agartala, Tripura, India.
Research Institute in Oncology & Hematology, Cancer Care Manitoba, Winnipeg, MB R3E OV9, Canada.
Toxicol In Vitro. 2019 Mar;55:24-32. doi: 10.1016/j.tiv.2018.11.004. Epub 2018 Nov 17.
The natural anti-cancer agent bromelain is found to be beneficial for either single or multi-targeted therapy in gastric and skin carcinoma, by inhibiting cancer cell growth. Importantly, the presence of peroxidase enhances its biological efficiency. We have now evaluated a panel of cancer cell lines with bromelain in presence or absence of peroxidase to identify that the combination has higher apoptosis inducing potential in all those cell lines. Bromelain plus peroxidase (BM-PR) inhibited acute myeloid (K562) cell proliferation and altered the morphological features. Incidence of apoptosis was established by using annexin V exposure and this was confirmed that the cell cycle was arrested at G0/G1 phase in a concentration-dependent manner. BM-PR increased the intracellular ROS level and altered the mitochondrial membrane potential, as detected using dichlorofluores cin diacetate (DCFDA). It also regulated the expression of apoptosis-related proteins like Bax, Bcl2, caspase-3 and cytochrome besides causing up-regulation of p53 as determined by western blot analysis. These results suggest that BM-PR from pineapple induces apoptosis better than only bromelain in acute myeloid leukemia cells possibly via mitochondria dependent pathway.
天然抗癌剂菠萝蛋白酶被发现通过抑制癌细胞生长，对胃癌和皮肤癌的单一或多靶点治疗都有益处。重要的是，过氧化物酶的存在增强了其生物学效率。我们现在已经评估了一组带有或不带有过氧化物酶的癌细胞系，以确定组合在所有这些细胞系中具有更高的凋亡诱导潜力。菠萝蛋白酶加过氧化物酶（BM-PR）抑制急性髓系（K562）细胞增殖并改变形态特征。通过使用膜联蛋白 V 暴露来确定细胞凋亡的发生率，这证实细胞周期以浓度依赖性方式在 G0/G1 期被阻滞。BM-PR 通过二氯荧光素二乙酸酯（DCFDA）检测到，增加了细胞内 ROS 水平并改变了线粒体膜电位。它还通过 Western blot 分析调节了凋亡相关蛋白的表达，如 Bax、Bcl2、caspase-3 和细胞色素 c，同时导致 p53 的上调。这些结果表明，菠萝中的 BM-PR 比单独的菠萝蛋白酶在急性髓系白血病细胞中诱导凋亡的效果更好，可能是通过线粒体依赖性途径。
