Department of Human Physiology, Tripura University, Immunology Microbiology Lab, Suryamaninagar, Tripura, India.
Department of Zoology, Tripura University, Suryamaninagar, Tripura, India.
Nutr Cancer. 2020;72(7):1200-1210. doi: 10.1080/01635581.2019.1670217. Epub 2019 Oct 8.
Pineapple ( (L.) Merr.) is a good source of bromelain (B) and also contain peroxidase. The objective of this study is isoaltion of bromelain plus peroxidase (BP) from the pineapple fruit to evaluate the anticancer activity of BP from the pineapple fruit of Tripura, compared to commercial bromelain against ascitic Dalton's lymphoma cells (DLA) in mice. By acetone precipitation BP was isolated from the pineapple. Animals bearing DLA, receive B and BP orally for 15 alternative days. Apoptotic proteins are assayed using western blot. BP treated mice showed recover of hemoglobin and WBC count compared to control lymphoma animal. The animal showed significant reduction of body weight due to reduced tunor load and elevated reactive oxygen species (ROS) production, elevated levels of vitamin C and vitamin E and other antioxidants in blood after BP treatment. Histology of liver and kidney also shows restored architecture in BP treated animal compared to only B treated group. BP treatment upregulates the cytochrome C, BAD, and BAX protein and downregulates the Bcl-2 and NF-kβ occuring upon BP treatment in the DLA cells collected from lymphoma animal. This induce the apoptosis of DLA cells in lymphoma animal and reduce the tumor load. The present findings suggest that BP from pineapple improves the survival of the induced lymphoma animal compared to only B which may be used as therapeutic target.
菠萝（(L.) Merr.）是一种很好的菠萝蛋白酶（B）来源，同时也含有过氧化物酶。本研究的目的是从菠萝果实中分离出菠萝蛋白酶加过氧化物酶（BP），并评估与商业菠萝蛋白酶相比，来自特里普拉邦菠萝果实的 BP 对小鼠腹水道尔顿淋巴瘤细胞（DLA）的抗癌活性。通过丙酮沉淀从菠萝中分离出 BP。患有 DLA 的动物口服接受 B 和 BP 治疗，共 15 天。使用 Western blot 检测凋亡蛋白。与对照淋巴瘤动物相比，BP 治疗的小鼠显示出血红蛋白和白细胞计数的恢复。由于肿瘤负荷减少和活性氧（ROS）产生增加，BP 治疗后的血液中维生素 C 和维生素 E 等抗氧化剂水平升高，动物体重显著减轻。肝和肾的组织学也显示出在 BP 治疗的动物中恢复了结构，而不仅仅是在 B 治疗的组中。BP 处理上调了 DLA 细胞中的细胞色素 C、BAD 和 BAX 蛋白，下调了 Bcl-2 和 NF-kβ，这在从淋巴瘤动物中收集的 DLA 细胞中发生。这诱导了淋巴瘤动物中 DLA 细胞的凋亡，减少了肿瘤负荷。本研究结果表明，与仅用 B 处理相比，来自菠萝的 BP 可提高诱导淋巴瘤动物的存活率，可作为治疗靶点。
