Ma S Y, Li C, Zhao H B, Zhang J Y, Zhang H Z, Wu K L, Li M
IVF Laboratory, Hospital for Reproductive Medicine Affiliated to Shandong University, Jinan 250021, China.
Zhonghua Fu Chan Ke Za Zhi. 2018 Nov 25;53(11):749-754. doi: 10.3760/cma.j.issn.0529-567x.2018.11.004.
To evaluate the application value of the blastocysts derived from non-pronucleus (0PN) zygotes by the good quality blastocyst formation rate and the clinical outcomes of frozen-thawed blastocyst transfers. The good quality blastocyst formation rate derived from 0PN zygotes was compared with that derived from2 pronucleus (2PN) zygotes in in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles from January 2015 to December 2016. In addition, the clinical pregnancy, embryo implantation and live birth rates of frozen-thawed blastocyst transfers with blastocysts derived from 0PN and 2PN zygotes were analyzed on corresponding dates. (1) In IVF cycles, the high quality blastocysts formation rate of 2PN embryos was significantly higher than that of 0PN (46.64% versus 42.42%, 0.01) . In ICSI cycles, the high quality blastocysts formation rate of 2PN embryos was markedly higher than that of 0PN (41.96% versus 21.73%, 0.01) . (2) In frozen-thawed embryo transfer cycles for IVF, the clinical pregnancy, implantation and live birth rates of D5 0PN blastocysts were significantly higher than those of D6 2PN (52.64% versus 46.78%, 49.91% versus 41.20%, 46.54% versus 39.56%, all 0.05) , however, the abortion and newborn abnormal rates of D5 0PN blastocysts were lower than those of D6 2PN blastocysts (17.37% versus 23.36%, 1.31% versus 4.21%, both 0.05) ; the clinical pregnancy, implantation and livebirth rates of D5 2PN blastocysts were significantly higher than those of D5 0PN (59.73% versus 52.64%, 55.95% versus 49.91%, 53.03% versus 46.54%, all 0.05) , but newborn abnormal rate was a little higher than that of D5 0PN (3.90% versus 1.31%, 0.05) ; the clinical pregnancy, implantation and live birth rates of D5 2PN blastocysts were significantly higher than those of D6 2PN (59.73% versus 46.78%, 55.95% versus 41.20%, 53.03% versus 39.56%, all 0.05) , and the abortion rate of D5 2PN blastocysts was lower than that of D6 2PN blastocysts (18.23% versus 23.36%, 0.05) . Although the blastocysts derived from 0PN could be transffered, the blastocysts derived from 2PN zygotes are preferred in all cycles. In IVF cycles, the good quality blastocysts derived from 2PN or 0PN zygotes will be transferred.
通过优质囊胚形成率及冻融囊胚移植的临床结局，评估非原核（0PN）受精卵来源囊胚的应用价值。比较2015年1月至2016年12月体外受精（IVF）或卵胞浆内单精子注射（ICSI）周期中，0PN受精卵与2原核（2PN）受精卵来源的优质囊胚形成率。此外，分析相应日期0PN和2PN受精卵来源囊胚冻融移植后的临床妊娠、胚胎着床及活产率。（1）在IVF周期中，2PN胚胎的优质囊胚形成率显著高于0PN（46.64%对42.42%，P<0.01）。在ICSI周期中，2PN胚胎的优质囊胚形成率明显高于0PN（41.96%对21.73%，P<0.01）。（2）在IVF的冻融胚胎移植周期中，D5期0PN囊胚的临床妊娠、着床及活产率显著高于D6期2PN囊胚（52.64%对46.78%，49.91%对41.20%，46.54%对39.56%，均P<0.05），然而，D5期0PN囊胚的流产及新生儿异常率低于D6期2PN囊胚（17.37%对23.36%，1.31%对4.21%，均P<0.05）；D5期2PN囊胚的临床妊娠、着床及活产率显著高于D5期0PN囊胚（59.73%对52.64%，55.95%对49.91%，53.03%对46.54%，均P<0.05），但新生儿异常率略高于D5期0PN囊胚（3.90%对1.31%，P<0.05）；D5期2PN囊胚的临床妊娠、着床及活产率显著高于D6期2PN囊胚（59.73%对46.78%，55.95%对41.20%，53.03%对39.56%，均P<0.05），且D5期2PN囊胚的流产率低于D6期2PN囊胚（18.23%对23.36%，P<0.05）。虽然0PN来源的囊胚可以移植，但在所有周期中2PN受精卵来源的囊胚更受青睐。在IVF周期中，2PN或0PN受精卵来源的优质囊胚均可移植。
