Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Eur J Obstet Gynecol Reprod Biol. 2021 Sep;264:206-211. doi: 10.1016/j.ejogrb.2021.07.033. Epub 2021 Jul 22.
To explore the development and pregnancy potential of non-pronuclear (0PN) zygote-derived embryos in conventional in vitro fertilization (IVF) cycles.
Embryonic development in 1039 oocyte retrieval cycles and clinical outcomes of 659 frozen-thawed blastocyst transfer cycles were retrospectively studied.
Developmental potential of embryos with different blastomere numbers on day 3 were inconsistent in 0PN and 2PN groups. For 0PN-derived embryos, blastocyst rate of fast developing embryos (75.4%) was similar to that of intermediately developing embryos (72.9%), but good quality blastocyst rate of the former (49.2%) was significantly higher than that of the later (39.6%). In 2PN group, intermediately developing embryos had the highest blastocyst rate (77.9%) and good quality blastocyst rate (51.5%) (statistically significant). Comparison of frozen-thawed transfer was carried out between 0PN- and 2PN-derived blastocysts. For both single (SBT) and double blastocyst transfer (DBT) groups, no statistical differences existed between 0PN- and 2PN-derived blastocysts in clinical pregnancy rates (45.2% and 49.1% in SBT group, 64.7% and 66.4% in DBT group), implantation rates (45.2% and 49.1% in SBT group, 41.2% and 47.7% in DBT group) and live birth rates (35.5% and 36.8% in SBT group, 52.9% and 51.2% in DBT group).
The developmental characteristic of 0PN-derived embryos was different from that of 2PN-derived embryos in IVF cycles. 0PN-derived blastocysts could obtain acceptable clinical pregnancy and live birth, but more studies are needed to confirm the safety..
探索常规体外受精（IVF）周期中非原核（0PN）受精卵胚胎的发育潜能和妊娠潜能。
回顾性研究了 1039 个卵母细胞采集周期的胚胎发育情况和 659 个冷冻解冻囊胚移植周期的临床结局。
在 0PN 和 2PN 组中，第 3 天具有不同卵裂球数目的胚胎的发育潜能不一致。对于 0PN 来源的胚胎，快速发育胚胎（75.4%）的囊胚率与中间发育胚胎（72.9%）相似，但前者（49.2%）的优质囊胚率明显高于后者（39.6%）。在 2PN 组中，中间发育胚胎的囊胚率最高（77.9%）和优质囊胚率（51.5%）（有统计学意义）。对 0PN 和 2PN 来源的囊胚进行了冷冻解冻移植比较。在单囊胚移植（SBT）和双囊胚移植（DBT）组中，0PN 和 2PN 来源的囊胚在临床妊娠率（SBT 组 45.2%和 49.1%，DBT 组 64.7%和 66.4%）、种植率（SBT 组 45.2%和 49.1%，DBT 组 41.2%和 47.7%）和活产率（SBT 组 35.5%和 36.8%，DBT 组 52.9%和 51.2%）方面均无统计学差异。
在 IVF 周期中，0PN 来源胚胎的发育特征与 2PN 来源胚胎不同。0PN 来源的囊胚可以获得可接受的临床妊娠和活产率，但需要更多的研究来确认其安全性。
