Asimakidou M, Oikonomou L, Filipopoulos A, Tsikopoulos G, Petropoulos A S
2Pediatric Surgery Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, , Greece.
Department of Histology and Embryology, School of Medicine, Aristotle University of Thessaloniki, , Greece.
Hippokratia. 2017 Apr-Jun;21(2):85-92.
Radiofrequency energy (RFE) has many medical applications in the treatment of adults and children. The impact of RFE on healing-regulation systems in the developing tissues is not fully known. Matrix metalloproteinases (MMPs) are involved in the remodeling of the extracellular matrix and the inflammatory processes. MMPs are regulated differently among the different age groups. We evaluated possible changes in MMP activity after an incisional wound using a radiofrequency scalpel in neonatal and adult rats.
In 30 Wistar rats [15 4-day-old (neonates) and 15 4-month-old (adults) rats], a ventral wound was created using a radiofrequency scalpel. Wounded areas and non-wounded tissues were harvested one, three and seven days after the intervention. Enzymatic activities of MMP-2 and MMP-9 were evaluated using gelatin zymography.
Adults expressed higher activity than neonates for MMP-2 on day 7 (Mann -Whitney U-test, p =0.009) and for MMP-9 on days one (p =0.005) and three (p =0.005). MMP-9 was expressed in higher amounts in the wounded tissue in comparison with non-wounded tissue during days one and three (Wilcoxon signed rank test, p =0.028 and p =0.043, respectively). MMP-2 was produced in equal amounts in the wounded and non-wounded tissue at all time-points. Only in the adult wounds at day seven, higher activity was noted compared with non-wounded skin (Wilcoxon signed rank test, p=0.043).
RFE, despite its local burning effect, does not interfere with known patterns of MMP regulation. Neonates have lower activity of MMPs than adults. Energy conduction through adjacent non-wounded tissues does not have an impact upon MMP regulation. HIPPOKRATIA 2017, 21(1): 85-92.
射频能量（RFE）在成人和儿童的治疗中有许多医学应用。RFE对发育中组织的愈合调节系统的影响尚不完全清楚。基质金属蛋白酶（MMPs）参与细胞外基质的重塑和炎症过程。MMPs在不同年龄组中的调节方式不同。我们评估了在新生大鼠和成年大鼠中使用射频手术刀造成切口伤口后MMP活性的可能变化。
在30只Wistar大鼠[15只4日龄（新生大鼠）和15只4月龄（成年大鼠）]中，使用射频手术刀造成腹部伤口。在干预后1天、3天和7天采集伤口部位和未受伤组织。使用明胶酶谱法评估MMP-2和MMP-9的酶活性。
在第7天，成年大鼠的MMP-2活性高于新生大鼠（曼-惠特尼U检验，p = 0.009），在第1天（p = 0.005）和第3天（p = 0.005），成年大鼠的MMP-9活性高于新生大鼠。在第1天和第3天，与未受伤组织相比，受伤组织中MMP-9的表达量更高（威尔科克森符号秩检验，分别为p = 0.028和p = 0.043）。在所有时间点，受伤组织和未受伤组织中MMP-2的产生量相等。仅在成年大鼠伤口的第7天，与未受伤皮肤相比，观察到更高的活性（威尔科克森符号秩检验，p = 0.043）。
尽管RFE有局部烧灼作用，但并不干扰已知的MMP调节模式。新生大鼠的MMP活性低于成年大鼠。通过相邻未受伤组织的能量传导对MMP调节没有影响。《希波克拉底》2017年，21(1): 85 - 92。
