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[具体研究对象]的转录组分析:非生物胁迫响应以及萜类和脂肪酸生物合成候选基因的揭示

Transcriptome Analysis of : A Revelation of Candidate Genes for Abiotic Stress Response and Terpenoid and Fatty Acid Biosyntheses.

作者信息

Zhang Xue, Zhang Yue, Wang Yue-Hua, Shen Shi-Kang

机构信息

School of Life Sciences, Yunnan University, Kunming, China.

出版信息

Front Genet. 2018 Nov 5;9:505. doi: 10.3389/fgene.2018.00505. eCollection 2018.

Abstract

, an endangered species endemic to Yunnan province, possesses large economic and phylogenetic values in Lauraceae. However, the genomic information of this species remains relatively unexplored. In this study, we used RNAseq technology to characterize and annotate the transcriptome and identify candidate genes involved in special metabolic pathways and gene-associated simple sequence repeats (SSRs) and single-nucleotide polymorphism (SNP). A total of 129,097 unigenes, with a mean length of 667 bp and an N50 length of 1,062 bp, were assembled. Among these genes, 56,887 (44.07%) unigenes were successfully annotated using at least one database. Furthermore, 47 and 46 candidate genes were identified in terpenoid biosynthesis and fatty acid biosynthesis, respectively. A total of 22 candidate genes participated in at least one abiotic stress response of . Additionally, a total of 25,654 SSRs and 640 SNPs were also identified. Based on these potential loci, 55 novel expressed sequence tag (EST)-SSR primers were successfully developed. This work provides comprehensive transcriptomic data that can be used to establish a valuable information platform for gene prediction, signaling pathway investigation, and molecular marker development for and other related species. Such a platform can facilitate further studies on germplasm conservation and utilization of Lauraceae species.

摘要

作为云南省特有的濒危物种,在樟科中具有重要的经济和系统发育价值。然而,该物种的基因组信息仍相对未被探索。在本研究中,我们使用RNA测序技术对转录组进行表征和注释,鉴定参与特殊代谢途径的候选基因以及与基因相关的简单序列重复(SSR)和单核苷酸多态性(SNP)。共组装了129,097个单基因,平均长度为667 bp,N50长度为1,062 bp。在这些基因中,56,887个(44.07%)单基因至少使用一个数据库成功注释。此外,分别在萜类生物合成和脂肪酸生物合成中鉴定出47个和46个候选基因。共有22个候选基因参与了至少一种非生物胁迫响应。此外,还鉴定出总共25,654个SSR和640个SNP。基于这些潜在位点,成功开发了55对新型表达序列标签(EST)-SSR引物。这项工作提供了全面的转录组数据,可用于为该物种及其他相关物种建立一个有价值的信息平台,用于基因预测、信号通路研究和分子标记开发。这样一个平台可以促进樟科物种种质资源保护和利用的进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f5c/6231050/529eddd187d9/fgene-09-00505-g001.jpg

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