人类白细胞抗原对哮喘发病的影响。

Effect of HLA on development of asthma.

作者信息

Mahdi Batool Mutar, Al-Hadithi Arwa Tahrir Ramadhan, Raouf Hyam, Zalzala Haider Hashim, Abid Laheeb Ali, Nehad Zena

机构信息

HLA research unit, Al-Kindy College of Medicine, University of Baghdad, Baghdad, Iraq.

Department of Microbiology, Al-Kindy College of Medicine, University of Baghdad, Baghdad, Iraq.

出版信息

Ann Med Surg (Lond). 2018 Oct 6;36:118-121. doi: 10.1016/j.amsu.2018.10.003. eCollection 2018 Dec.

DOI:10.1016/j.amsu.2018.10.003

PMID:30455876

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6230961/

Abstract

BACKGROUND

Asthma is a disease characterized by a chronic inflammation of the airways caused by the interaction of genetic susceptibility with environmental factors. Inflammation and remodeling are critical components of asthma. It is shown that many genes are involved in the pathogenesis of asthma.

AIM OF STUDY

To identify/compare the association between HLA-DRB1 alleles and development of asthma in a sample of Iraqi Arab Muslims.

PATIENTS AND METHODS

A case-control study (forty patients and forty healthy control) was carried out in Medical city Teaching Hospital, Baghdad-Iraq. The study participants were patients with asthma consulted the hospital from September - 2013 to January - 2015. HLA -DRB1genotyping was done using a panel of sequence-specific oligonucleotide probes (SSOP) using HLA-DRB1amplification and hybridization kits (SSO HLA type DRB1 plus and Mastermix for HLA type DRB1 Amp plus kits -Innogenetics-Belgium) using automated method by AutoLipa - 48Innogenetics-Belgum.

RESULTS

There was an increased frequency of HLADRB103:01:01 in control group compared with patients group (P = 0.009, Odds ratio = 0.1228, 95% CI: 0.0254-0.5930). Other allele like HLA-DRB1 070101was significantly increased in asthmatic patients in compares with control group (P = 0.005, Odds ratio = 6.641, 95% CI: 1.7319-25.4657).

CONCLUSIONS

HLA alleles have an effect on development asthma in patients with HLA-DRB1070101 while HLADRB1 030101 may have a protective effect in Iraqi Arab Muslims individuals against development of asthma.

摘要

背景

哮喘是一种由遗传易感性与环境因素相互作用导致气道慢性炎症的疾病。炎症和重塑是哮喘的关键组成部分。研究表明，许多基因参与哮喘的发病机制。

研究目的

在伊拉克阿拉伯穆斯林样本中识别/比较HLA - DRB1等位基因与哮喘发病之间的关联。

患者与方法

在伊拉克巴格达医学城教学医院开展了一项病例对照研究（40例患者和40例健康对照）。研究参与者为2013年9月至2015年1月期间到该医院就诊的哮喘患者。使用HLA - DRB1扩增和杂交试剂盒（SSO HLA DRB1 plus和用于HLA DRB1 Amp plus试剂盒的Mastermix - Innogenetics - 比利时），通过AutoLipa - 48 Innogenetics - 比利时的自动化方法，使用一组序列特异性寡核苷酸探针（SSOP）进行HLA - DRB1基因分型。

结果

与患者组相比，对照组中HLADRB103:01:01的频率增加（P = 0.009，优势比 = 0.1228，95%置信区间：0.0254 - 0.5930）。与对照组相比，哮喘患者中其他等位基因如HLA - DRB1070101显著增加（P = 0.005，优势比 = 6.641，95%置信区间：1.7319 - 25.4657）。