Samappito Jatupat, Yamada Mamoru, Klanrit Preekamol, Thanonkeo Pornthap
1Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002 Thailand.
2Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515 Japan.
3 Biotech. 2018 Nov;8(11):474. doi: 10.1007/s13205-018-1493-7. Epub 2018 Nov 9.
A thermoadapted strain of designated ZM AD41 that capable of growth and ethanol production at high temperature was obtained using the thermal stress adaptation technique. This thermoadapted strain exhibited approximately 1.8 and 27fold higher growth rate than the wildtype at 39 °C and 41 °C, respectively. It was more resistant to stress induced by acetic acid at 200 mM and hydrogen peroxide (HO) at 0.4 mM and produced approximately 1.8 and 38.6fold higher ethanol concentrations than the wildtype at 39 °C and 41 °C, respectively. Moreover, it had better sedimentation performance during ethanol fermentation at high temperature than the wildtype. Based on the growth performance, heat, acetic acid and HO stress treatments, sedimentation characteristics, and ethanol fermentation capability, ZM AD41 was a good candidate for ethanol production at high temperature.
使用热应激适应技术获得了一株命名为ZM AD41的热适应菌株，该菌株能够在高温下生长并产生乙醇。在39°C和41°C时，该热适应菌株的生长速率分别比野生型高约1.8倍和27倍。它对200 mM乙酸和0.4 mM过氧化氢（H₂O₂）诱导的应激更具抗性，在39°C和41°C时产生的乙醇浓度分别比野生型高约1.8倍和38.6倍。此外，在高温乙醇发酵过程中，它比野生型具有更好的沉降性能。基于生长性能、热、乙酸和H₂O₂应激处理、沉降特性以及乙醇发酵能力，ZM AD41是高温乙醇生产的良好候选菌株。
