Farjadian Fatemeh, Azadi Soha, Mohammadi-Samani Soliman, Ashrafi Hajar, Azadi Amir
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Clinical Pharmacy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Heliyon. 2018 Nov 12;4(11):e00930. doi: 10.1016/j.heliyon.2018.e00930. eCollection 2018 Nov.
Mesoporous silica with hexagonal type structure containing amine functional group was introduced. Firstly, aminopropyl hexagonal mesoporous silica was synthetized in a co-condensation process, via templating route of -dodecylamine. Then synthesized mesoporous material were characterized, and FT-IR spectrum confirmed the presence of amine group and CHN analysis determined the amount of organic layer. The high surface area (750 m/g) was determined by applying nitrogen adsorption-desorption technique. The morphology was examined by scanning electron microscopy which proved hexagonal structure. The crystallinity of mesoporous material was observed in XRD pattern of this material. According to previous background of such material in adsorbing drug, herein, the efficiency of this material in adsorbing of 5-fluorouracil was evaluated through solid phase extraction method in aqueous and plasma media with high performance liquid chromatography. The extraction efficiency was evaluated for drug concentrations of 500-2000 ng/ml by means of 5-20 mg/ml hexagonal mesoporous silica in both media. The results showed good to excellent recovery rate of in both aqueous and plasma medium which confirmed that the aminopropyl functionalized hexagonal mesoporous silica could be considered as promising device for drug bioanalysis.
引入了具有六边形结构且含有胺官能团的介孔二氧化硅。首先，通过十二烷基胺的模板路线，在共缩合过程中合成了氨丙基六边形介孔二氧化硅。然后对合成的介孔材料进行表征，傅里叶变换红外光谱（FT-IR）证实了胺基的存在，元素分析（CHN）确定了有机层的含量。通过应用氮气吸附-脱附技术测定了高比表面积（750 m²/g）。通过扫描电子显微镜检查形态，证实了六边形结构。在该材料的X射线衍射（XRD）图谱中观察到介孔材料的结晶度。根据此类材料在吸附药物方面的先前背景，在此，通过高效液相色谱法在水相和血浆介质中采用固相萃取法评估了该材料吸附5-氟尿嘧啶的效率。在两种介质中，通过5-20 mg/ml的六边形介孔二氧化硅对500-2000 ng/ml的药物浓度评估了萃取效率。结果表明，在水相和血浆介质中均具有良好至优异的回收率，这证实了氨丙基官能化的六边形介孔二氧化硅可被视为用于药物生物分析的有前景的装置。
