Cha Sung-Jae, McLean Kyle Jarrod, Jacobs-Lorena Marcelo
Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Life Sci Alliance. 2018 Sep 18;1(5):e201800111. doi: 10.26508/lsa.201800111. eCollection 2018 Oct.
sporozoite liver infection is an essential step for parasite development in its mammalian host. Previously, we used a phage display library to identify mimotope peptides that bind to Kupffer cells and competitively inhibit sporozoite-Kupffer cell interaction. These peptides led to the identification of a Kupffer cell receptor-CD68-and a sporozoite ligand-GAPDH-that are required for sporozoite traversal of Kupffer cells and subsequent infection of hepatocytes. Here, we report that the C-terminal end of GAPDH interacts with the Kupffer CD68 receptor, and identify two epitopes within this region as candidate antigens for the development of antibodies that inhibit infection.
子孢子肝感染是寄生虫在其哺乳动物宿主体内发育的关键步骤。此前，我们利用噬菌体展示文库鉴定出与库普弗细胞结合并竞争性抑制子孢子与库普弗细胞相互作用的模拟表位肽。这些肽促使我们鉴定出库普弗细胞受体-CD68-以及子孢子配体-GAPDH-，它们是子孢子穿过库普弗细胞并随后感染肝细胞所必需的。在此，我们报告GAPDH的C末端与库普弗细胞CD68受体相互作用，并鉴定出该区域内的两个表位作为开发抑制感染抗体的候选抗原。
