Wang Zhi-Gang, Lv Nan, Bi Wen-Zhi, Zhang Ji-Lin, Ni Jia-Zuan
†State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
‡University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
ACS Appl Mater Interfaces. 2015 Apr 29;7(16):8377-92. doi: 10.1021/acsami.5b01254. Epub 2015 Apr 16.
Reversible protein phosphorylation is a key event in numerous biological processes. Mass spectrometry (MS) is the most powerful analysis tool in modern phosphoproteomics. However, the direct MS analysis of phosphorylated proteins/peptides is still a big challenge because of the low abundance and insufficient ionization of phosphorylated proteins/peptides as well as the suppression effects of nontargets. Enrichment of phosphorylated proteins/peptides by affinity materials from complex biosamples is the most widely used strategy to enhance the MS detection. The demand of efficiently enriching phosphorylated proteins/peptides has spawned diverse affinity materials based on different enrichment principles (e.g., electronic attraction, chelating). In this review, we summarize the recent development of various affinity materials for phosphorylated proteins/peptides enrichment. We will highlight the design and fabrication of these affinity materials, discuss the enrichment mechanisms involved in different affinity materials, and suggest the future challenges and research directions in this field.
可逆蛋白磷酸化是众多生物过程中的关键事件。质谱（MS）是现代磷酸蛋白质组学中最强大的分析工具。然而，由于磷酸化蛋白质/肽丰度低、电离不足以及非靶标的抑制作用，对磷酸化蛋白质/肽进行直接质谱分析仍然是一个巨大挑战。通过亲和材料从复杂生物样品中富集磷酸化蛋白质/肽是增强质谱检测最广泛使用的策略。高效富集磷酸化蛋白质/肽的需求催生了基于不同富集原理（如电子吸引、螯合）的多种亲和材料。在本综述中，我们总结了用于富集磷酸化蛋白质/肽的各种亲和材料的最新进展。我们将重点介绍这些亲和材料的设计与制备，讨论不同亲和材料所涉及的富集机制，并提出该领域未来的挑战和研究方向。
