Laboratório de Genética Evolutiva Paulo Leminsk, Departamento de Biologia Molecular, Centro de Ciencias Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, Brazil.
J Mol Evol. 2019 Jan;87(1):7-15. doi: 10.1007/s00239-018-9877-1. Epub 2018 Nov 20.
The definition of a genomic signature (GS) is "the total net response to selective pressure". Recent isolation and sequencing of naturally occurring organisms, hereby named entoorganisms, within Acanthamoeba polyphaga, raised the hypothesis of a common genomic signature despite their diverse and unrelated evolutionary origin. Widely accepted and implemented tests for GS detection are oligonucleotide relative frequencies (OnRF) and relative codon usage (RCU) surveys. A common pattern and strong correlations were unveiled from OnRFs among A. polyphaga's Mimivirus and virophage Sputnik. RCU showed a common A-T bias at third codon position. We expanded tests to the amoebal mitochondrial genome and amoeba-resistant bacteria, achieving strikingly coherent results to the aforementioned viral analyses. The GSs in these entoorganisms of diverse evolutionary origin are coevolutionarily conserved within an intracellular environment that provides sanctuary for species of ecological and biomedical relevance.
基因组特征（GS）的定义是“对选择压力的总净反应”。最近在多形艾美耳球虫内分离和测序了自然发生的生物体，即内生生物体，提出了尽管它们具有不同的和无关的进化起源，但存在共同基因组特征的假说。广泛接受和实施的 GS 检测方法是寡核苷酸相对频率（OnRF）和相对密码子使用（RCU）调查。在多形艾美耳球虫的 mimivirus 和 virophage Sputnik 之间的 OnRFs 中揭示了一种共同的模式和强烈的相关性。RCU 在第三个密码子位置显示出共同的 A-T 偏向。我们将测试扩展到阿米巴线粒体基因组和阿米巴抗性细菌，得到了与上述病毒分析惊人一致的结果。在提供生态和生物医学相关物种庇护的细胞内环境中，这些具有不同进化起源的内生生物体的 GS 是共同进化保守的。
