使用抗猫血小板反应蛋白单克隆抗体PMab-52检测虎血小板反应蛋白。
Detection of Tiger Podoplanin Using the Anti-Cat Podoplanin Monoclonal Antibody PMab-52.
作者信息
Yamada Shinji, Itai Shunsuke, Furusawa Yoshikazu, Sano Masato, Nakamura Takuro, Yanaka Miyuki, Handa Saori, Hisamatsu Kayo, Nakamura Yoshimi, Fukui Masato, Harada Hiroyuki, Mizuno Takuya, Sakai Yusuke, Ogasawara Satoshi, Murata Takeshi, Uchida Hiroaki, Tahara Hideaki, Kaneko Mika K, Kato Yukinari
机构信息
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
2 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
出版信息
Monoclon Antib Immunodiagn Immunother. 2018 Nov;37(5):224-228. doi: 10.1089/mab.2018.0033.
Podoplanin (PDPN) is expressed in type I alveolar cells of lung but not in type II alveolar cells. PDPN is also known as a specific lymphatic endothelial cell marker because PDPN is not expressed in vascular endothelial cells. PDPNs of several animals have been characterized using specific anti-PDPN monoclonal antibodies (mAbs): PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 for mouse, rat, rabbit, dog, bovine, and cat PDPNs, respectively. In this study, we investigated the possible crossreaction between these anti-PDPN mAbs and tiger PDPN. Flow cytometry and western blot analyses revealed that the anti-cat PDPN mAb PMab-52 (IgM, kappa) reacted with tiger PDPN, which is overexpressed in Chinese hamster ovary-K1 cells. Using immunohistochemical analysis, type I alveolar cells of the tiger lung were strongly detected by PMab-52. These results indicate that PMab-52 may be useful for the detection of tiger PDPN.
血小板源性生长因子结合蛋白（PDPN）在肺的I型肺泡细胞中表达，但在II型肺泡细胞中不表达。PDPN也被称为一种特异性淋巴管内皮细胞标志物，因为它在血管内皮细胞中不表达。几种动物的PDPN已使用特异性抗PDPN单克隆抗体（mAb）进行了表征：分别用于小鼠、大鼠、兔子、狗、牛和猫PDPN的PMab-1、PMab-2、PMab-32、PMab-38、PMab-44和PMab-52。在本研究中，我们研究了这些抗PDPN mAb与老虎PDPN之间可能的交叉反应。流式细胞术和蛋白质印迹分析表明，抗猫PDPN mAb PMab-52（IgM，κ）与在中国仓鼠卵巢-K1细胞中过表达的老虎PDPN发生反应。使用免疫组织化学分析，PMab-52强烈检测到老虎肺的I型肺泡细胞。这些结果表明，PMab-52可能有助于检测老虎PDPN。
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