Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan.
Monoclon Antib Immunodiagn Immunother. 2020 Jun;39(3):91-94. doi: 10.1089/mab.2020.0008. Epub 2020 May 14.
Monoclonal antibodies (mAbs) that specifically target podoplanin (PDPN), a marker for type I alveolar cells, are needed for immunohistochemical analyses. Anti-PDPN mAbs are available for many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, Tasmanian devil, alpaca, tiger, whale, goat, horse, bear, and sheep PDPNs. However, no antilion PDPN (lioPDPN) antibody has been developed. In this study, possible cross-reaction between available anti-PDPN mAbs and lioPDPN was examined. Immunohistochemical analysis showed that antitiger PDPN mAb PMab-231 (IgG, kappa) reacted with type I alveolar cells from lion lung, indicating that PMab-231 is useful for the detection of lioPDPN.
单克隆抗体（mAbs）特异性靶向 podoplanin（PDPN），是一种Ⅰ型肺泡细胞的标志物，用于免疫组织化学分析。针对 PDPN 的 mAbs 可用于许多物种，包括人、小鼠、大鼠、兔、狗、猫、牛、猪、塔斯马尼亚恶魔、羊驼、虎、鲸、山羊、马、熊和绵羊的 PDPNs。然而，尚未开发出针对狮子 PDPN（lioPDPN）的抗体。在这项研究中，检查了现有的抗 PDPN mAbs 与 lioPDPN 之间可能的交叉反应。免疫组织化学分析表明，抗虎 PDPN mAb PMab-231（IgG，kappa）与来自狮子肺的Ⅰ型肺泡细胞反应，表明 PMab-231 可用于检测 lioPDPN。
