Sleibi A, Tappuni A, Mills D, Davis G R, Baysan A
Oper Dent. 2018 Nov/Dec;43(6):E308-E316. doi: 10.2341/18-014-L.
: The objective of this in vitro study was to quantify the amount of mineral change in demineralized dentin at pH 5.5 after the application of dental varnishes containing fluoride with casein phosphopeptide-amorphous calcium phosphate, fluoride and bioglass, or fluoride alone.
: A total of 12 extracted human sound mandibular premolar root samples were coated with an acid-resistant varnish, leaving a 2 × 3 mm window at the outer root surface. These root specimens were then randomly divided into four groups and separately subjected to the demineralizing cycle at a pH of 4.8 for five days to create artificial caries-like lesions in dentin. Subsequently, each sample was imaged using quantitative x-ray microtomography (XMT) at a 15-μm voxel size. Each test group then received one of the following treatments: dental varnish containing casein phosphopeptide-amorphous calcium phosphate and fluoride (CPP-ACP, MI varnish, GC Europe), bioglass and fluoride (BGA, Experimental, Dentsply Sirona), or fluoride alone (NUPRO, Dentsply Sirona), as well as a control group, which received no treatment. These samples were kept in deionized water for 12 hours. The thin layer of varnish was then removed. All samples including the nonvarnish group were subjected to the second demineralizing cycle at pH 5.5 for five days. The final XMT imaging was then carried out following the second demineralizing cycle. XMT scan was also carried out to varnish samples at 25 μm voxel size. The change in mineral concentration in the demineralized teeth was assessed using both qualitative and quantitative image analysis.
: There was an increase in radiopacity in the subtracted images of all varnish groups; a significant increase in mineral content, 12% for the CPP-ACP and fluoride ( p≤0.05 and p≤0.001), 25% BGA ( p≤0.001), and 104% fluoride alone varnish ( p≤0.001). There was an increase in the size of radiolucency in the lesion area with a significant decrease in mineral content in the nonvarnish group, 10% ( p≤0.05 and p≤0.001).
: There was encouraging evidence of a remineralization effect following the application of dental varnish on dentin and also an observed resistance to demineralization during the acidic challenge in all cases. However, a dental varnish containing fluoride alone appeared to have a much greater effect on dentin remineralization when compared with CPP-ACP with fluoride and bioglass with fluoride.
本体外研究的目的是量化在应用含氟与酪蛋白磷酸肽-无定形磷酸钙、氟化物和生物玻璃的牙科清漆或仅含氟化物的牙科清漆后，pH值为5.5时脱矿牙本质中的矿物质变化量。
总共12个拔除的健康人下颌前磨牙牙根样本用耐酸清漆包被，在外牙根表面留一个2×3毫米的窗口。然后将这些牙根标本随机分为四组，并分别在pH值为4.8的条件下进行脱矿循环5天，以在牙本质中形成人工龋样病变。随后，每个样本使用体素大小为15微米的定量X射线显微断层扫描（XMT）进行成像。然后每个测试组接受以下治疗之一：含酪蛋白磷酸肽-无定形磷酸钙和氟化物的牙科清漆（CPP-ACP，MI清漆，GC欧洲公司）、生物玻璃和氟化物（BGA，实验性，登士柏西诺德公司）或仅含氟化物（NUPRO，登士柏西诺德公司），以及一个未接受治疗的对照组。这些样本在去离子水中保存12小时。然后去除清漆薄层。所有样本包括未涂清漆组在pH值为5.5的条件下进行第二个脱矿循环5天。在第二个脱矿循环后进行最终的XMT成像。还以25微米体素大小对涂有清漆的样本进行XMT扫描。使用定性和定量图像分析评估脱矿牙齿中矿物质浓度的变化。
所有清漆组的相减图像中的不透射线性增加；矿物质含量显著增加，含CPP-ACP和氟化物的为12%（p≤0.05和p≤0.001），BGA为25%（p≤0.001），仅含氟化物清漆的为104%（p≤0.001）。未涂清漆组病变区域的透射线性大小增加，矿物质含量显著降低，为10%（p≤0.05和p≤0.001）。
有令人鼓舞的证据表明，在牙本质上应用牙科清漆后有再矿化作用，并且在所有情况下在酸性挑战期间都观察到对脱矿的抵抗力。然而，与含氟化物的CPP-ACP和含氟化物的生物玻璃相比，仅含氟化物的牙科清漆对牙本质再矿化的作用似乎要大得多。
