Polansky Ondrej, Seidlerova Zuzana, Faldynova Marcela, Sisak Frantisek, Rychlik Ivan
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Vet Immunol Immunopathol. 2018 Nov;205:10-16. doi: 10.1016/j.vetimm.2018.10.006. Epub 2018 Oct 21.
Events occurring in the chicken caecum following Salmonella Enteritidis infection are relatively well-described. However, mechanisms of the immune response and defence beyond the intestinal tract are less well-described. In this study, we therefore determined changes in protein abundance in the liver and blood serum in response to S. Enteritidis infection using the unbiased approach of shotgun proteomics. Complement and coagulation cascades, TNF signalling, antigen processing and presentation was activated in the liver following infection with S. Enteritidis. Chicken proteins that decreased in the liver were involved in glycolysis, the citrate cycle, oxidative phosphorylation and fatty acid metabolism. No functional category was significantly activated or suppressed in the serum. Concerning individual proteins, VNN1, SAA, AVD, SERPINA3, SERPINB10, AGT, MRP126 or CP increased in abundance both in the liver and serum. MT4, MT3, PTGDS, GLRX and TGM4, though highly inducible in the liver, did not increase in the serum. PIGR, SERPINF2 and IGJ increased in the serum but not in the liver. SERPINA4, apoAIV, CLEC3B, SERPINF1, HRG, AHSG and ALB decreased both in the liver and serum. Avidin-like LOC431660, THRSP, GATM, GGACT, ACOX1, ALDOB or FABP7 decreased in the liver but not in the serum. Finally, CKM, CKB, PLTP, COMP, IGFALS, AMY1A or SERPIND1 decreased in the serum after S. Enteritidis infection but not in the liver. Differently abundant proteins characterise the chicken's response to infection and can be also used as markers of chicken health status.
肠炎沙门氏菌感染后鸡盲肠中发生的事件已有较为详尽的描述。然而，肠道外免疫反应和防御机制的描述则较少。因此，在本研究中，我们采用鸟枪法蛋白质组学的无偏方法，确定了肠炎沙门氏菌感染后肝脏和血清中蛋白质丰度的变化。感染肠炎沙门氏菌后，肝脏中的补体和凝血级联反应、TNF信号传导、抗原加工和呈递被激活。肝脏中减少的鸡蛋白质参与糖酵解、柠檬酸循环、氧化磷酸化和脂肪酸代谢。血清中没有功能类别被显著激活或抑制。关于单个蛋白质，VNN1、SAA、AVD、SERPINA3、SERPINB10、AGT、MRP126或CP在肝脏和血清中的丰度均增加。MT4、MT3、PTGDS、GLRX和TGM4虽然在肝脏中高度可诱导，但在血清中并未增加。PIGR、SERPINF2和IGJ在血清中增加，但在肝脏中未增加。SERPINA4、载脂蛋白AIV、CLEC3B、SERPINF1、HRG、AHSG和ALB在肝脏和血清中均减少。类抗生物素蛋白样LOC431660、THRSP、GATM、GGACT、ACOX1、ALDOB或FABP7在肝脏中减少，但在血清中未减少。最后，肠炎沙门氏菌感染后，血清中的CKM、CKB、PLTP、COMP、IGFALS、AMY1A或SERPIND1减少，但在肝脏中未减少。不同丰度的蛋白质表征了鸡对感染的反应，也可作为鸡健康状况的标志物。
