Li Xiaocheng, Zhang Peng, Jiang Xiaosong, Du Huarui, Yang Chaowu, Zhang Zengrong, Men Shuai, Zhang Zhikun, Jiang Wei, Wang Hongning
College of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern, Chengdu 610065, China; Sichuan Academy of Animal Sciences, Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu 610066, China.
College of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern, Chengdu 610065, China.
Vet Immunol Immunopathol. 2017 Jul;189:28-35. doi: 10.1016/j.vetimm.2017.05.003. Epub 2017 May 11.
Salmonella enterica serovar (S. enteritidis) is a pathogenic bacterium that can cause symptoms of food poisoning, leading to death of poultry, resulting in serious economic losses. The MyD88 and TRIF signalling pathways play important roles in activating innate and adaptive immunity in chickens infected with S. enteritidis. The objective of the present study was to characterize in vivo mRNA expressions, protein levels and methylation levels of genes in the above two pathways in both Tibetan chickens and DaHeng S03 chickens infected with S. enteritidis. MyD88-dependent and TRIF-dependent signalling pathway were activated by infection, and the MyD88 signalling pathway induced cytokines LITAF and IL-8 played important roles in fighting against the S. enteritidis infection in vivo. The TLR4 methylation might alter expression of genes involved in the MyD88 signalling pathway, and thus different breeds of chickens might show differences in susceptibility to the S. enteritidis. The increased expression of INF β was activated by S. enteritidis, but its expressions were different in levels of mRNA and protein in Tibetan chickens and DaHeng chickens, suggesting its functions on the resistance to S. enteritidis infection in chickens. This study contributes to the understanding of two pathways activated in response to S. enteritidis infection, and gives indications on the mechanisms underlying resistance of Tibetan chickens and DaHeng chickens to S. enteritidis.
肠炎沙门氏菌血清型（肠炎沙门氏菌）是一种致病细菌，可引起食物中毒症状，导致家禽死亡，造成严重的经济损失。MyD88和TRIF信号通路在感染肠炎沙门氏菌的鸡激活先天免疫和适应性免疫中发挥重要作用。本研究的目的是表征感染肠炎沙门氏菌的藏鸡和大恒S03鸡上述两条通路中基因的体内mRNA表达、蛋白质水平和甲基化水平。感染激活了MyD88依赖性和TRIF依赖性信号通路，MyD88信号通路诱导的细胞因子LITAF和IL-8在体内抵抗肠炎沙门氏菌感染中发挥重要作用。TLR4甲基化可能改变MyD88信号通路中相关基因的表达，因此不同品种的鸡对肠炎沙门氏菌的易感性可能存在差异。肠炎沙门氏菌激活了INFβ表达的增加，但其在藏鸡和大恒鸡中的mRNA和蛋白质水平表达不同，表明其在鸡抵抗肠炎沙门氏菌感染中的作用。本研究有助于理解肠炎沙门氏菌感染后激活的两条通路，并揭示藏鸡和大恒鸡对肠炎沙门氏菌抗性的潜在机制。
