Zhang Hao, Yue Mengxia, Zheng Xueke, Gautam Mayank, He Shibin, Li Lijia
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
State Key Laboratory of Cotton Biology, College of Life Sciences, Henan University, Kaifeng, China.
Front Plant Sci. 2018 Oct 16;9:1520. doi: 10.3389/fpls.2018.01520. eCollection 2018.
In plants, lateral roots play a crucial role in the uptake of water and nutrients. Several genes such as () and () have been found to be involved in lateral root development. In the present investigation, we observed that heat treatment might be involved in the inhibition of lateral root primordium (LRP) formation in maize, accompanied by an increase in global acetylation levels of histone 3 lysine residue 9 (H3K9) and histone 4 lysine residue 5 (H4K5), suggesting that histone modification was related to LRP inhibition. However, Trichostatin A (TSA), an inhibitor of histone deacetylases (HDACs), apparently did not inhibit the LRP formation, revealing that global hyperacetylation might not be the determining factor in the LRP inhibition induced by heat stress. Furthermore, expression of genes related to lateral root development in maize, and , was down-regulated and the acetylation levels in the promoter region of these two genes were decreased under heat stress, suggesting that promoter-associated histone acetylation might be associated with the expression of and genes which were found to be involved in the heat-induced LRP inhibition in maize.
在植物中，侧根在水分和养分吸收方面发挥着关键作用。已发现诸如（）和（）等几个基因参与侧根发育。在本研究中，我们观察到热处理可能参与了玉米侧根原基（LRP）形成的抑制，同时伴随着组蛋白3赖氨酸残基9（H3K9）和组蛋白4赖氨酸残基5（H4K5）的整体乙酰化水平升高，这表明组蛋白修饰与LRP抑制有关。然而，组蛋白去乙酰化酶（HDACs）抑制剂曲古抑菌素A（TSA）显然并未抑制LRP形成，这表明整体高乙酰化可能不是热胁迫诱导LRP抑制的决定性因素。此外，玉米中与侧根发育相关的基因和的表达在热胁迫下被下调，并且这两个基因启动子区域的乙酰化水平降低，这表明启动子相关的组蛋白乙酰化可能与和基因的表达有关，已发现这两个基因参与了玉米热诱导的LRP抑制。
