Effects of Diet on the Gut Bacterial Community of (Diptera: Calliphoridae) across Developmental Stages.

The blowfly, (Diptera: Calliphoridae), is a well-known forensically important insect. Basic data related to have been well documented; but despite the pivotal role of gut microbes in various facets of insect biology, little is known about its gut microbiome. To investigate the gut bacterial community of and explore its stability, diet and developmental stage were selected as the two variables. Larvae were reared on bovine liver, swine manure, and chicken manure, and high-throughput sequencing of the 1st, 2nd, and 3rd instar larvae and the newly emerged adults was performed. According to our results, the alpha diversity of the gut bacterial community did not significantly vary among different diets and developmental stages. Principal coordinate analysis revealed that the gut microbiome of clustered together among different diets and developmental stages. The main phyla in the gut microbial community of were Firmicutes and Proteobacteria, and the dominant genera were , , , and . These findings characterized the gut microbiome of and demonstrated that the gut bacterial community is fairly stable. The dominant genera , , , and have the potential to serve as core microbiomes within the gut microbial community of .