Li Tian, Ma Hongyun, Peng Yan, Chen Xiaohong, Zhu Zhi, Wu Xu, Kou Tianyi, Song Bin, Guo Shiwei, Liu Lizhuang, Zhu Yiming
Terahertz Technology Innovation Research Institute, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
Department of Pancreatic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China.
Biomed Opt Express. 2018 Oct 16;9(11):5467-5476. doi: 10.1364/BOE.9.005467. eCollection 2018 Nov 1.
Homocysteine is an amino acid related to metabolism in human vivo, which is closely related to cardiovascular disease, senile dementia, bone fracture, et al. Currently, the usual medical test methods for homocysteine include high performance liquid chromatography (HPLC), fluorescence polarization immunoassay (FPIA) and enzyme-linked immunosorbent assay (ELISA), which are time-consuming or expensive. In this paper, we first analyze the vibration and rotation of homocysteine molecules by using density functional theory, and then we ensure that the theoretical absorption peaks are located in the range of the terahertz spectrum. Then, based on the terahertz time-domain spectroscopy system, we measured the absorption spectrum of homocysteine under different concentrations. It is found that as the detection of the concentration, the terahertz results present higher accuracy than that of the laser Raman spectrum, which can be used as the reference for the evaluation of pathological stage. These results are of great significance for the exact and quick diagnosis of homocysteine-related diseases in clinical medicine.
同型半胱氨酸是人体内与新陈代谢相关的一种氨基酸，它与心血管疾病、老年痴呆症、骨折等密切相关。目前，检测同型半胱氨酸的常用医学检测方法包括高效液相色谱法（HPLC）、荧光偏振免疫分析法（FPIA）和酶联免疫吸附测定法（ELISA），这些方法耗时或昂贵。在本文中，我们首先利用密度泛函理论分析同型半胱氨酸分子的振动和转动，然后确定理论吸收峰位于太赫兹光谱范围内。接着，基于太赫兹时域光谱系统，我们测量了不同浓度下同型半胱氨酸的吸收光谱。研究发现，随着浓度检测，太赫兹结果比激光拉曼光谱具有更高的准确性，可作为评估病理阶段的参考。这些结果对于临床医学中同型半胱氨酸相关疾病的准确快速诊断具有重要意义。
