Jung Gyeong Bok, Huh Jeong-Eun, Lee Hyo-Jung, Kim Dohyun, Lee Gi-Ja, Park Hun-Kuk, Lee Jae-Dong
Department of Physics Education, Chosun University, Gwangju, 61452, South Korea.
These authors contributed equally to this work.
Biomed Opt Express. 2018 Oct 25;9(11):5703-5718. doi: 10.1364/BOE.9.005703. eCollection 2018 Nov 1.
We demonstrated the apoptotic effect of bee venom (BV) on human MDA-MB-231 breast cancer cells using Raman spectroscopy and principal component analysis (PCA). Biochemical changes in cancer cells were monitored following BV treatment; the results for different concentrations and treatment durations differed markedly. Significantly decreased Raman vibrations for DNA and proteins were observed for cells treated with 3.0 µg/mL BV for 48 h compared with those of control cells. These results suggest denaturation and degradation of proteins and DNA fragmentation (all cell death-related processes). The Raman spectroscopy results agreed with those of atomic force microscopy and conventional biological tests such as viability, TUNEL, and western blot assays. Therefore, Raman spectroscopy, with PCA, provides a noninvasive, label-free tool for assessment of cellular changes on the anti-cancer effect of BV.
我们使用拉曼光谱和主成分分析（PCA）证明了蜂毒（BV）对人MDA - MB - 231乳腺癌细胞的凋亡作用。在BV处理后监测癌细胞的生化变化；不同浓度和处理持续时间的结果有显著差异。与对照细胞相比，用3.0μg/mL BV处理48小时的细胞中，DNA和蛋白质的拉曼振动显著降低。这些结果表明蛋白质变性和降解以及DNA片段化（所有与细胞死亡相关的过程）。拉曼光谱结果与原子力显微镜以及传统生物学测试（如活力、TUNEL和蛋白质免疫印迹分析）的结果一致。因此，拉曼光谱结合PCA为评估BV抗癌作用引起的细胞变化提供了一种非侵入性、无标记的工具。
