Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland.
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Lublin, Poland.
PLoS One. 2018 Nov 21;13(11):e0207793. doi: 10.1371/journal.pone.0207793. eCollection 2018.
Probiotics improve consumers' health and additionally may positively influence the microbiological and organoleptic quality of food. In the study, legume sprouts were inoculated with Lactobacilllus plantarum 299V to produce a new functional product ensuring the growth and survival of the probiotic and high microbiological quality of the final product. Legume sprouts, which are an excellent source of nutrients, were proposed as alternative carriers for the probiotic. The key factors influencing the production of probiotic-rich sprouts include the temperature (25°C) of sprouting and methods of inoculation (soaking seeds in a suspension of probiotics). Compared to the control sprouts, the sprouts enriched with the probiotic were characterized by lower mesophilic bacterial counts. In the case of fresh and stored probiotic-rich sprouts, lactic acid bacteria (LAB) accounted for a majority of total microorganisms. The Lb. plantarum population was also stable during the cold storage. The high count of LAB observed in the digest confirmed the fact that the studied sprouts are effective carriers for probiotics and ensure their survival in the harmful conditions of the digestive tract in an in vitro model. Enrichment of legume sprouts with probiotics is a successful attempt and yields products for a new branch of functional foods.
益生菌可改善消费者的健康状况,还可能积极影响食品的微生物学和感官质量。在这项研究中,将植物乳杆菌 299V 接种到豆科芽苗菜中,以生产一种新的功能性产品,确保益生菌的生长和存活以及最终产品的高微生物质量。豆科芽苗菜是营养的极好来源,被提议作为益生菌的替代载体。影响富含益生菌芽苗菜生产的关键因素包括发芽温度(25°C)和接种方法(将种子浸泡在益生菌悬浮液中)。与对照芽苗菜相比,用益生菌富集的芽苗菜的兼性需氧菌计数较低。在新鲜和储存的富含益生菌的芽苗菜中,乳酸菌(LAB)占总微生物的大多数。在冷藏过程中,Lb. plantarum 种群也保持稳定。在消化物中观察到的高 LAB 计数证实了一个事实,即研究中的芽苗菜是益生菌的有效载体,并确保它们在体外模型中在消化道的有害条件下存活。用益生菌富集豆科芽苗菜是一次成功的尝试,并生产出功能性食品的一个新分支的产品。