Hernández-Gómez Jimmy G, López-Bonilla Argelia, Trejo-Tapia Gabriela, Ávila-Reyes Sandra V, Jiménez-Aparicio Antonio R, Hernández-Sánchez Humberto
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, UP Adolfo López Mateos, Mexico City CP 07738, Mexico.
Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec CP 62731, Mexico.
Foods. 2021 Mar 22;10(3):674. doi: 10.3390/foods10030674.
Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast , the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.
益生菌菌株中的胆汁盐水解酶（BSH）活性通常与降低高胆固醇血症患者血清胆固醇水平的能力相关。本研究的目的是评估五株乳酸菌（LAB）益生菌菌株和一株益生菌酵母中的BSH。使用定性直接平板试验和定量高效薄层色谱分析法评估活性。这六株菌株在BSH底物偏好和活性方面存在差异。DGIA1是从墨西哥恰帕斯州的一种双层奶油奶酪中分离出的潜在益生菌菌株，在四种测试胆汁酸中表现出优异的去结合活性（甘氨胆酸钠、甘氨脱氧胆酸钠、牛磺胆酸钠和牛磺脱氧胆酸钠的去结合活性分别为69%、100%、81%和92%）。对于市售的益生菌酵母，其对甘氨脱氧胆酸钠、牛磺胆酸钠和牛磺脱氧胆酸钠的去结合活性良好（分别为100%、57%和63%）。最后这两个结果是该研究工作的新颖之处。在甘氨胆酸钠的情况下观察到较弱的去结合活性（5%）。这是首次在这种酵母中检测到BSH活性。
