Nasrabadi Niyousha, Soheili Zahra-Soheila, Bagheri Abouzar, Ahmadieh Hamid, Amizadeh Yashar, Sahebjam Farzin, Tabeie Faraj, Rezaei Kanavi Mozhgan
Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Bioelectromagnetics. 2018 Dec;39(8):585-594. doi: 10.1002/bem.22154.
A great deal of evidence has confirmed that electromagnetic fields (EMFs) can affect the central nervous system. In this study, cultured neonatal human retinal pigment epithelial (hRPE) cells were exposed to pulsed EMF of 1 mT intensity and 50 Hz frequency 8 h daily for 3 days. In addition to cell proliferation and cell death assays, immunocytochemistry for RPE65, PAX6, nestin, and cytokeratin 8/18 proteins were performed. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for NES, PAX6, RPE65, and ACTA2 gene expression. Exposed hRPE cells did not demonstrate significant change in terms of cytomorphology, cell proliferation, or cell death. Protein expression of PAX6 was decreased in treated cells compared to controls and remained unchanged for RPE65, cytokeratin 8/18, and nestin. Gene expressions of NES, RPE65, and PAX6 were decreased in treated cells as compared to controls. Gene expression of ACTA2 did not significantly change. In conclusion, viability of cultivated neonatal hRPE cells did not change after short exposure to a safe dose of pulsed EMF albeit that both gene and protein expressions of retinal progenitor cell markers were reduced. Whether longer exposure durations that are being constantly produced by widely-used electronic devices may induce significant changes in these cells, needs further investigation. Bioelectromagnetics. 39:585-594, 2018. © 2018 Wiley Periodicals, Inc.
大量证据证实，电磁场（EMF）可影响中枢神经系统。在本研究中，将培养的新生人类视网膜色素上皮（hRPE）细胞每天暴露于强度为1 mT、频率为50 Hz的脉冲电磁场8小时，持续3天。除了进行细胞增殖和细胞死亡检测外，还对RPE65、PAX6、巢蛋白和细胞角蛋白8/18蛋白进行了免疫细胞化学检测。对NES、PAX6、RPE65和ACTA2基因表达进行了实时逆转录聚合酶链反应（RT-PCR）。暴露的hRPE细胞在细胞形态、细胞增殖或细胞死亡方面未显示出显著变化。与对照组相比，处理细胞中PAX6的蛋白表达降低，而RPE65、细胞角蛋白8/18和巢蛋白的表达保持不变。与对照组相比，处理细胞中NES、RPE65和PAX6的基因表达降低。ACTA2的基因表达没有显著变化。总之，短期暴露于安全剂量的脉冲电磁场后，培养的新生hRPE细胞的活力没有改变，尽管视网膜祖细胞标志物的基因和蛋白表达均降低。广泛使用的电子设备持续产生的更长暴露时间是否会在这些细胞中引起显著变化，需要进一步研究。生物电磁学。39:585 - 594, 2018。© 2018威利期刊公司。
