Oladnabi Morteza, Mishan Mohammad Amir, Rezaeikanavi Mozhgan, Zargari Mehryar, Sadeghi Rouhallah Najjar, Bagheri Abouzar
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
J Ophthalmic Vis Res. 2021 Apr 29;16(2):202-211. doi: 10.18502/jovr.v16i2.9084. eCollection 2021 Apr-Jun.
Emerging evidence implies that electromagnetic fields (EMFs) can negatively affect angiogenesis. In this regard, the effects of extremely low frequency pulsed electromagnetic field (ELF-PEMF) exposure on the relative expression level of angiogenic factors involved in the pathogenesis of ocular disorders were evaluated in human retinal pigment epithelial (hRPE) cells in order to investigate a noninvasive therapeutic method for patients with several ocular diseases associated with neovascularization.
After separating hRPE cells from globes, hRPE cells were exposed to 15 mT of ELF-PEMF (120 Hz) at 5, 10, and 15 min for seven days. Cell proliferation and apoptosis of treated cells were evaluated via ELISA assay. Moreover, relative expression changes of HIF-1α, CTGF, VEGFA, MMP-2, cathepsin D, and E2F3 were performed using real-time RT-PCR.
ELF-PEMF exposure had no significant effects on the apoptosis and proliferation rate of hRPE cells. Expression level of HIF-1α, CTGF, VEGFA, MMP-2, cathepsin D, and E2F3 was downregulated following 5 min of ELF-PEMF exposure.
As ELF-PEMF showed inhibitory effects on the expression of angiogenic genes in hRPE cells with no cytotoxic or proliferative side effects, it can be introduced as a useful procedure for managing angiogenesis induced by retinal pathogenesis, although more studies with adequate follow-up in animal models are needed.
新出现的证据表明，电磁场（EMF）可对血管生成产生负面影响。在这方面，为了研究一种针对几种与新生血管形成相关的眼部疾病患者的非侵入性治疗方法，在人视网膜色素上皮（hRPE）细胞中评估了极低频脉冲电磁场（ELF-PEMF）暴露对参与眼部疾病发病机制的血管生成因子相对表达水平的影响。
从眼球分离出hRPE细胞后，将hRPE细胞暴露于15 mT的ELF-PEMF（120 Hz）下，分别暴露5、10和15分钟，持续7天。通过ELISA检测评估处理后细胞的增殖和凋亡情况。此外，使用实时RT-PCR检测HIF-1α、CTGF、VEGFA、MMP-2、组织蛋白酶D和E2F3的相对表达变化。
ELF-PEMF暴露对hRPE细胞的凋亡和增殖率没有显著影响。ELF-PEMF暴露5分钟后，HIF-1α、CTGF、VEGFA、MMP-2、组织蛋白酶D和E2F3的表达水平下调。
由于ELF-PEMF对hRPE细胞中血管生成基因的表达具有抑制作用，且无细胞毒性或增殖副作用，因此可作为一种管理视网膜发病机制诱导的血管生成的有用方法引入，尽管还需要在动物模型中进行更多有充分随访的研究。
