Institute of Chemistry, Federal University of Mato Grosso do Sul, Av. Senador Filinto Muller, 1555, CP 549, CEP 79074-460 Campo Grande, MS, Brazil.
School of Physical Sciences, National Centre for Plasma Science and Technology, Dublin City University, Glasnevin, Dublin 9, Ireland.
Sci Total Environ. 2019 Feb 15;651(Pt 2):2845-2856. doi: 10.1016/j.scitotenv.2018.10.173. Epub 2018 Oct 13.
CuO nanostructured thin films supported on silicon with 6.5 cm area (geometric area greater than the studies reported in the literature) were synthesized by a chemical bath deposition technique. The electrodes were characterized by MEV, XRD, XPS, contact angle, cyclic voltammetry and electrochemical impedance spectroscopy analyses. To evaluate the photoelectrochemical properties of the CuO films, photocurrent-voltage measurements were performed using linear voltammetry. The catalytic activities of CuO nanostructures were evaluated by monitoring photodegradation of Mitoxantrone (MTX) under UV-A light irradiation. The method of photoelectrocatalysis (PEC), applying a voltage of 1.5 V and assisted by adding HO, was undertaken. To the best of our knowledge, no studies on the degradation of anticancer agents using PEC process have been found in the literature. For comparison purposes, experiments were performed under the same conditions by assisted photocatalysis (PC) with HO and direct photolysis. CuO deposits consist of a needle-like morphology. The presence of CuO in the tenorite phase was evidenced by XRD and the XPS spectra showed the presence of copper(II) oxide. The increase in current under illumination shows that CuO exhibits photoactivity. The PEC system showed a 75% level of MTX degradation, while the level achieved using PC was 50%. Under UV-A light alone only 3% removal was obtained after 180 min. Up to 10 by-products were identified by chromatography-mass spectrometry (LC-MS) with m/z values ranging between 521 and 285 and a plausible degradation route has been proposed. It is worth mentioning that 9 by-products identified in this work, were not found in the literature in other studies of degradation or products generated as metabolites. The toxicity tests of MTX before and after PEC treatment with Artemia Salina and Allium cepa showed a decrease in the acute toxicity of the medium as the antineoplastic was degraded.
在硅衬底上制备了面积为 6.5cm²（比文献中报道的面积更大）的氧化铜纳米结构薄膜，采用化学浴沉积技术合成。通过 MEV、XRD、XPS、接触角、循环伏安法和电化学阻抗谱分析对电极进行了表征。为了评估 CuO 薄膜的光电化学性质，通过线性伏安法进行了光电流-电压测量。通过监测在 UV-A 光照射下米托蒽醌（MTX）的光降解，评估了 CuO 纳米结构的催化活性。采用施加 1.5V 电压的光电催化（PEC）方法，并通过添加 HO 辅助进行。据我们所知，文献中尚未发现使用 PEC 工艺降解抗癌药物的研究。为了进行比较，在相同条件下进行了 HO 辅助光催化（PC）和直接光解的实验。CuO 沉积物呈针状形态。XRD 证实了纤锌矿相的 CuO 存在，XPS 光谱显示存在氧化铜（II）。光照下电流的增加表明 CuO 具有光活性。PEC 系统显示 MTX 降解率达到 75%，而使用 PC 时的降解率为 50%。单独在 UV-A 光下，180 分钟后仅去除了 3%。通过色谱-质谱联用（LC-MS）鉴定了多达 10 种副产物，其 m/z 值在 521 至 285 之间，并提出了一种可能的降解途径。值得一提的是，在这项工作中鉴定的 9 种副产物，在其他降解研究或作为代谢产物生成的产物中，在文献中没有找到。使用 Artemia Salina 和 Allium cepa 对 PEC 处理前后的 MTX 进行的毒性测试表明，随着抗肿瘤药物的降解，介质的急性毒性降低。
