Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China.
Theor Appl Genet. 2019 Feb;132(2):543-553. doi: 10.1007/s00122-018-3241-0. Epub 2018 Nov 21.
Cultivated rice (Oryza sativa) originated from common wild rice (Oryza rufipogon) and inherited its advantages. However, during the rice domestication process, some valuable features of wild rice, such as tolerance to biotic and abiotic stress, were lost. To fully utilize wild rice germplasm resources, we constructed a set of introgression lines (ILs) using a common wild rice material from Lingshui, China. A set of high-resolution InDel molecular markers with an average interval of 2.39 Mb were designed to carry out marker-assisted selection and identification of segment characteristics. The ILs contained 77 lines including 1.286 introgressed fragments with an average length of 6.511 Mb, covering 93.59% of the donor parent's chromosomes. The agricultural traits of 77 lines were investigated. Many old quantitative trait loci (QTLs) involved in plant height, awn length, seed traits and other characteristics reappeared in our ILs, proving that our system was reliable. Further, many new QTLs were identified. A QTL related to drought tolerance located on chromosome 4 was thoroughly elaborated. This set of ILs provides a new resource for utilizing the excellent features of wild rice.
栽培稻（Oryza sativa）起源于普通野生稻（Oryza rufipogon），并继承了其优势。然而，在水稻驯化过程中，野生稻的一些有价值的特征，如对生物和非生物胁迫的耐受性，丢失了。为了充分利用野生稻种质资源，我们利用来自中国陵水的一种普通野生稻材料构建了一套导入系（ILs）。设计了一套平均间隔为 2.39 Mb 的高分辨率 InDel 分子标记，用于进行标记辅助选择和片段特征鉴定。这些 ILs 包含 77 条系，包含 1.286 个平均长度为 6.511 Mb 的导入片段，涵盖了供体亲本染色体的 93.59%。对 77 条系的农业性状进行了研究。许多涉及株高、芒长、种子特性等特征的旧数量性状位点（QTLs）在我们的 ILs 中重新出现，证明了我们的系统是可靠的。此外，还鉴定了许多新的 QTLs。对位于 4 号染色体上与耐旱性相关的一个 QTL 进行了详细阐述。这组 ILs 为利用野生稻的优良特性提供了新的资源。
