National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
National Center for Gene Research, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Nat Genet. 2015 Jul;47(7):827-33. doi: 10.1038/ng.3305. Epub 2015 May 18.
Global warming threatens many aspects of human life, for example, by reducing crop yields. Breeding heat-tolerant crops using genes conferring thermotolerance is a fundamental way to help deal with this challenge. Here we identify a major quantitative trait locus (QTL) for thermotolerance in African rice (Oryza glaberrima), Thermo-tolerance 1 (TT1), which encodes an α2 subunit of the 26S proteasome involved in the degradation of ubiquitinated proteins. Ubiquitylome analysis indicated that OgTT1 protects cells from heat stress through more efficient elimination of cytotoxic denatured proteins and more effective maintenance of heat-response processes than achieved with OsTT1. Variation in TT1 has been selected for on the basis of climatic temperature and has had an important role in local adaptation during rice evolution. In addition, we found that overexpression of OgTT1 was associated with markedly enhanced thermotolerance in rice, Arabidopsis and Festuca elata. This discovery may lead to an increase in crop security in the face of the ongoing threat of global warming.
全球变暖威胁到人类生活的许多方面，例如，它会降低作物产量。使用赋予耐热性的基因来培育耐热作物是应对这一挑战的根本方法。在这里，我们确定了一个非洲稻（Oryza glaberrima）耐热性的主要数量性状位点（QTL），称为耐热性 1（TT1），它编码参与泛素化蛋白降解的 26S 蛋白酶体的 α2 亚基。泛素组分析表明，OgTT1 通过更有效地消除细胞毒性变性蛋白和更有效地维持热响应过程来保护细胞免受热应激，这比 OsTT1 更有效。TT1 的变异是基于气候温度选择的，在水稻进化过程中的局部适应中发挥了重要作用。此外，我们发现 TT1 的过表达与水稻、拟南芥和羊茅中耐热性的显著增强有关。这一发现可能会增加作物的安全性，以应对全球变暖带来的持续威胁。
