Unité de Biochimie Macromoléculaire et Génétique, Faculté des Sciences de Gafsa, cité Zarroug, Université de Gafsa, 2112, Gafsa, Tunisia.
Unité de Physiologie Intégrée, Laboratoire de Pathologies Vasculaires, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Jarzouna, Tunisia.
Environ Sci Pollut Res Int. 2020 Feb;27(6):5651-5660. doi: 10.1007/s11356-018-3771-7. Epub 2018 Nov 21.
The purpose of this study was to investigate, for the first time, the effects of Bifenthrin (Bif) chronic exposure on plasmatic and aortic lipid parameters disturbance and their pro-atherogenic possibility in Wistar rats. The ameliorative role of vitamin E (Vit E) and selenium (Se) were also targeted. Thus, rats were treated by gastric gavage with combination of Vit E (100 mg/kg/bw) and Se (0.25 mg/kg/bw) in alone and co-treated groups for 90 days. Apart from control and Vit E-Se groups, all the groups were subjected to Bif (3 mg/kg, via gavage) toxicity. Results showed that Bif increased markedly plasmatic and aortic total cholesterol, LDL-cholesterol, native LDL-apoB-100, and oxidized-LDL, compared to the control. Moreover, Bif treatment significantly increased the plasmatic levels of the pro-inflammatory cytokines TNF-α, IL-2, and IL-6. In addition, the densitometric quantification of protein bands showed that the amount of hepatic native LDL-receptor protein decreased significantly in the intoxicated rats compared to the control group. The expression of arterial LDL receptors (LDLRs) and scavenger receptors (CD36) was amplified owing to Bif toxicity. This harmful effect was confirmed by histological study using Oil-Red-O staining. Owing to their antioxidant capacities, Vit E and Se have maintained all the changes in plasma and aorta lipids and prevented the pro-atherogenic effect observed in Bif-treated animals.
本研究首次旨在探讨联苯菊酯（Bif）慢性暴露对 Wistar 大鼠血浆和主动脉脂质参数紊乱及其致动脉粥样硬化的可能性，并研究维生素 E（Vit E）和硒（Se）的改善作用。因此，大鼠通过胃灌胃用 Vit E（100mg/kg/bw）和 Se（0.25mg/kg/bw）单独和联合治疗组处理 90 天。除了对照组和 Vit E-Se 组外，所有组都接受了联苯菊酯（3mg/kg，通过灌胃）毒性。结果表明，与对照组相比，Bif 显著增加了血浆和主动脉总胆固醇、LDL-胆固醇、天然 LDL-apoB-100 和氧化 LDL。此外，Bif 处理显著增加了促炎细胞因子 TNF-α、IL-2 和 IL-6 的血浆水平。此外，蛋白条带的密度定量显示，与对照组相比，中毒大鼠肝脏天然 LDL 受体蛋白的量显著减少。动脉 LDL 受体（LDLRs）和清道夫受体（CD36）的表达因 Bif 毒性而放大。这一有害影响通过用油红 O 染色进行的组织学研究得到证实。由于其抗氧化能力，Vit E 和 Se 维持了血浆和主动脉脂质的所有变化，并防止了在 Bif 处理的动物中观察到的致动脉粥样硬化作用。
