Shinohara Yasutomo, Nishimura Ikuko, Koyama Yasuji
a Noda Institute for Scientific Research , Noda City , Chiba Prefecture , Japan.
Biosci Biotechnol Biochem. 2019 Aug;83(8):1506-1513. doi: 10.1080/09168451.2018.1549934. Epub 2018 Nov 22.
Heptelidic acid (HA), a sesquiterpene lactone, is a known inhibitor of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Recently, we found that HA was produced by RIB40 and acted as the growth inhibitor of the salt-tolerant lactic acid bacteria in soy sauce brewing. Although several decades have passed since the discovery of HA, the genes involved in its biosynthesis and biosynthetic pathway have not yet been fully identified. In this study, we identified the HA biosynthetic gene cluster (HA cluster) using gene disruption and expression analysis. We also revealed that two transcription regulatory genes adjacent to the HA cluster were responsible for the expression of HA biosynthetic genes in . Interestingly, the HA cluster contained a gene encoding GAPDH (), which showed much higher resistance to HA than the GAPDH gene () located at the other locus, but which did not seem to act as a self-resistant gene.
庚二烯酸（HA）是一种倍半萜内酯，是已知的甘油醛-3-磷酸脱氢酶（GAPDH）抑制剂。最近，我们发现HA由RIB40产生，并在酱油酿造中作为耐盐乳酸菌的生长抑制剂。尽管自HA被发现以来已经过去了几十年，但其生物合成相关基因及其生物合成途径尚未完全确定。在本研究中，我们通过基因敲除和表达分析鉴定了HA生物合成基因簇（HA簇）。我们还发现，与HA簇相邻的两个转录调控基因负责HA生物合成基因在[具体位置]的表达。有趣的是，HA簇包含一个编码GAPDH的基因（[具体基因名称]），该基因对HA的抗性远高于位于其他位点的GAPDH基因（[具体基因名称]），但似乎并不作为自身抗性基因起作用。
