Fredens K, Dybdahl H, Dahl R, Baandrup U
Department of Neurobiology, University of Aarhus, Denmark.
APMIS. 1988 Aug;96(8):711-9. doi: 10.1111/j.1699-0463.1988.tb00934.x.
In a series of eosinophil inflammatory states affecting various organs (heart, gut, bladder and skin) we performed an immunohistochemical study of the eosinophil cationic proteins ECP and EPX. A strong correlation was noted between the liberation of ECP and EPX and tissue necrosis in all organs. In most cases ECP and EPX were found on the same location. However, one case indicated a possible differential release. Extracellular ECP and EPX were revealed concurrently with the two polyclonal antibodies and the monoclonal EG2 antibody. The latter binds to both ECP and EPX, but only during secretion. Since EG2 does not differentiate between ECP and EPX, but only during secretion. Since EG2 does not differentiate between EXP and EPX, it is for the first time demonstrated that both cationic proteins are correlated to tissue damage. The chymotrypsin-like cationic protein (CCP), related to neutrophils, showed a low correlation with the eosinophil cationic proteins in cases of tissue damage. The hypothesis is put forward that the release of eosinophil granule proteins and especially ECP results in a non-specific tissue damage.
在一系列影响不同器官（心脏、肠道、膀胱和皮肤）的嗜酸性粒细胞炎症状态中，我们对嗜酸性粒细胞阳离子蛋白ECP和EPX进行了免疫组织化学研究。在所有器官中，均发现ECP和EPX的释放与组织坏死之间存在强烈相关性。在大多数情况下，ECP和EPX位于相同位置。然而，有一个病例显示可能存在差异释放。使用两种多克隆抗体和单克隆EG2抗体同时检测到细胞外ECP和EPX。后者仅在分泌过程中与ECP和EPX结合。由于EG2在分泌过程中无法区分ECP和EPX，因此首次证明这两种阳离子蛋白均与组织损伤相关。与中性粒细胞相关的类胰凝乳蛋白酶阳离子蛋白（CCP）在组织损伤病例中与嗜酸性粒细胞阳离子蛋白的相关性较低。我们提出假说，即嗜酸性粒细胞颗粒蛋白尤其是ECP的释放会导致非特异性组织损伤。
