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拟南芥 DGAT3 是一种参与 TAG 生物合成的 [2Fe-2S] 蛋白。

Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis.

机构信息

Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000, Versailles, France.

Laboratoire de Chimie des Processus Biologiques, UMR 8229 CNRS, Collège de France, Université Paris 6, 11 Place Marcelin Berthelot, 75231, Paris, CEDEX 05, France.

出版信息

Sci Rep. 2018 Nov 22;8(1):17254. doi: 10.1038/s41598-018-35545-7.

DOI:10.1038/s41598-018-35545-7
PMID:30467384
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6250708/
Abstract

Acyl-CoA:diacylglycerol acyltransferases 3 (DGAT3) are described as plant cytosolic enzymes synthesizing triacylglycerol. Their protein sequences exhibit a thioredoxin-like ferredoxin domain typical of a class of ferredoxins harboring a [2Fe-2S] cluster. The Arabidopsis thaliana DGAT3 (AtDGAT3; At1g48300) protein is detected in germinating seeds. The recombinant purified protein produced from Escherichia coli, although very unstable, exhibits DGAT activity in vitro. A shorter protein version devoid of its N-terminal putative chloroplast transit peptide, Δ46AtDGAT3, was more stable in vitro, allowing biochemical and spectroscopic characterization. The results obtained demonstrate the presence of a [2Fe-2S] cluster in the protein. To date, AtDGAT3 is the first metalloprotein described as a DGAT.

摘要

酰基辅酶 A：二酰基甘油酰基转移酶 3（DGAT3）被描述为植物细胞质酶，可合成三酰基甘油。它们的蛋白质序列表现出硫氧还蛋白样铁氧还蛋白结构域，这是一类具有[2Fe-2S]簇的铁氧还蛋白的典型特征。拟南芥 DGAT3（AtDGAT3；At1g48300）蛋白在萌发的种子中被检测到。尽管从大肠杆菌中纯化得到的重组蛋白非常不稳定，但在体外具有 DGAT 活性。一种缺少其 N 端假定的质体转运肽的较短蛋白版本，Δ46AtDGAT3，在体外更稳定，允许进行生化和光谱学表征。获得的结果表明该蛋白中存在[2Fe-2S]簇。迄今为止，AtDGAT3 是第一个被描述为 DGAT 的金属蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/09334819f74e/41598_2018_35545_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/3889fec2dd0b/41598_2018_35545_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/ada9f3fbcb81/41598_2018_35545_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/079dce3c9551/41598_2018_35545_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/193a9fb61bd9/41598_2018_35545_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/41a78f102894/41598_2018_35545_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/09334819f74e/41598_2018_35545_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/3889fec2dd0b/41598_2018_35545_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/ada9f3fbcb81/41598_2018_35545_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/079dce3c9551/41598_2018_35545_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/193a9fb61bd9/41598_2018_35545_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/41a78f102894/41598_2018_35545_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/016f/6250708/09334819f74e/41598_2018_35545_Fig6_HTML.jpg

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