Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
Harbin Academy of Agricultural Science, Harbin 150028, China.
Int J Mol Sci. 2023 Feb 17;24(4):4078. doi: 10.3390/ijms24044078.
Hempseed is a nutrient-rich natural resource, and high levels of hempseed oil accumulate within hemp seeds, consisting primarily of different triglycerides. Members of the diacylglycerol acyltransferase (DGAT) enzyme family play critical roles in catalyzing triacylglycerol biosynthesis in plants, often governing the rate-limiting step in this process. As such, this study was designed to characterize the () gene family in detail. Genomic analyses of the revealed 10 candidate genes that were classified into four families (, , , ) based on the features of different isoforms. Members of the family were found to be associated with large numbers of cis-acting promoter elements, including plant response elements, plant hormone response elements, light response elements, and stress response elements, suggesting roles for these genes in key processes such as development, environmental adaptation, and abiotic stress responses. Profiling of these genes in various tissues and varieties revealed varying spatial patterns of expression dynamics and differences in expression among varieties, suggesting that the members of this gene family likely play distinct functional regulatory functions genes were upregulated in response to cold stress, and significant differences in the mode of regulation were observed when comparing roots and leaves, indicating that genes may play positive roles as regulators of cold responses in hemp while also playing distinct roles in shaping the responses of different parts of hemp seedlings to cold exposure. These data provide a robust basis for further functional studies of this gene family, supporting future efforts to screen the significance of candidate genes to validate their functions to improve hempseed oil composition.
麻籽是一种营养丰富的天然资源，大量的麻籽油积聚在麻籽中，主要由不同的三酰基甘油组成。二酰基甘油酰基转移酶（DGAT）酶家族的成员在植物中催化三酰基甘油的生物合成中起着关键作用，通常控制着这个过程的限速步骤。因此，本研究旨在详细描述 （）基因家族。对 进行基因组分析，揭示了 10 个候选 基因，根据不同同工型的特征将它们分为四个家族（、、、）。 家族的成员与大量顺式作用启动子元件相关，包括植物反应元件、植物激素反应元件、光反应元件和应激反应元件，表明这些基因在发育、环境适应和非生物胁迫反应等关键过程中发挥作用。对这些基因在不同组织和品种中的表达谱进行分析，揭示了 表达动力学的空间模式存在差异，不同品种之间的表达也存在差异，表明该基因家族的成员可能在发育、环境适应和非生物胁迫反应等关键过程中发挥独特的功能调节作用。 基因在冷胁迫下上调，根和叶之间观察到调控模式的显著差异，表明 基因可能在麻中作为冷响应的正调控因子发挥作用，同时在塑造麻幼苗不同部位对冷暴露的响应方面也发挥着不同的作用。这些数据为进一步研究该基因家族的功能提供了坚实的基础，支持未来筛选 候选基因的重要性，以验证其功能，从而改善麻籽油的成分。
