Muthulakshmi Kannaiyan, Uma Chinnaiyan
Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu 608002, India.
Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu 608002, India,
Front Biosci (Elite Ed). 2019 Jan 1;11(1):89-101. doi: 10.2741/E848.
We tested the hypothesis that silver nanoparticles (BsAgNPs) made by subsp. exhibit antibacterial and antifungal activities. The bacteria formed nanoparticles in UV-visible spectra with the peak around 430 nanometer detected by surface plasmon resonance and Lambdamax. The FTIR spectra, SEM and XRD revealed the involvement of interaction of biological moieties in the formation of crystalline cuboidal nanoparticles that were had an average size of 10-20 nm. The particles exerted dose dependent antibacterial and antifungal activities and high dose equivalent to standard antibiotics. Minimum inhibitory concentration, minimum bactericidal and fungicidal concentrations were in the range from 3.1-25 microgram/ml and 6.3-50 microgram/ml, respectively. BsAgNPs significantly inhibited the growth of multiple strains including multidrug resistant (MDR) bacteria. Moreover, the combination of BsAgNPs and antibiotics showed synergistic inhibition of MDR strains. These data show that BsAgNPs have a great potential in the treatment of MDR bacteria without or with standard antibiotics.
由亚种制备的银纳米颗粒（BsAgNPs）具有抗菌和抗真菌活性。细菌在紫外可见光谱中形成纳米颗粒，通过表面等离子体共振和最大吸收波长检测到其峰值约为430纳米。傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和X射线衍射（XRD）表明，生物部分的相互作用参与了平均尺寸为10 - 20纳米的立方体形结晶纳米颗粒的形成。这些颗粒具有剂量依赖性的抗菌和抗真菌活性，高剂量时等同于标准抗生素。最低抑菌浓度、最低杀菌和杀真菌浓度分别在3.1 - 25微克/毫升和6.3 - 50微克/毫升范围内。BsAgNPs显著抑制了包括多重耐药（MDR）细菌在内的多种菌株的生长。此外，BsAgNPs与抗生素的组合对MDR菌株表现出协同抑制作用。这些数据表明，BsAgNPs在单独或与标准抗生素联合治疗MDR细菌方面具有巨大潜力。
