Department of Energy and Materials, Konkuk University, Chungju 27478, Republic of Korea.
Department of Energy and Materials, Konkuk University, Chungju 27478, Republic of Korea.
Biosens Bioelectron. 2019 Feb 1;126:381-388. doi: 10.1016/j.bios.2018.11.012. Epub 2018 Nov 13.
This research demonstrated the electrochemical modification of low-cost titanium (Ti) metal substrate with gold nanoparticles (AuNPs) for the aptamer-based detection of cardiac troponin I (cTnI). AuNPs were deposited onto Ti sheets by the potential-step deposition method with high density and homogeneity as well as good crystallinity. It was then applied as a transducer to immobilize a thiol-functionalized DNA aptamer via the self-assembled monolayer mechanism for the specific binding of cTnI. This was verified through electrochemical and morphological analyses. The aptasensor could detect cTnI in a linear range of 1-1100 pM with a detection limit of ca. 0.18 pM. The aptasensor showed high sensitivity and specificity to cTnI over other interfering compounds with good recoveries in the diluted human serum samples.
本研究展示了通过电化学方法在低成本钛（Ti）金属基底上修饰金纳米粒子（AuNPs），用于基于适配体的心肌肌钙蛋白 I（cTnI）检测。通过恒电位沉积法在 Ti 片上沉积 AuNPs，具有高密度、均一性和良好的结晶度。然后，它被用作传感器，通过自组装单层机制固定巯基化 DNA 适配体，用于 cTnI 的特异性结合。这通过电化学和形态分析得到了验证。该适配体传感器可在 1-1100 pM 的线性范围内检测 cTnI，检测限约为 0.18 pM。该适配体传感器对 cTnI 具有高灵敏度和特异性，对其他干扰化合物的检测具有良好的回收率，可用于稀释人血清样本的检测。
