Department of Biomedical Engineering, Kyungil University, 50 Gamasil-gil, Hayang-eup, Gyeongsan-si, Gyeongbuk-do 38428, Korea.
Sensors (Basel). 2018 Nov 22;18(12):4084. doi: 10.3390/s18124084.
Despite its broad penetration of various markets, the quantitative lateral flow immunoassay (LFIA) suffers from sensitivity issues in some cases. To solve this problem, an optimized colorimetric readout method for LFIA quantification is proposed in this study. An assay reader device utilizing a color camera and an analysis method using a Bayer filtered image were developed. Spectrometric measurements of the assay test line were performed to determine the color channel that contains the test line information and effectively minimizes noise. The change in the intensity ratio with increasing concentration of the target substance in the sample was largest in the green channel. The linear range of the output curve ranged from 0 to 10 ng/mL, and the detection limit was 2 ng/mL. The suggested instrumentation and analysis methods are expected to effectively resolve the low-sensitivity problems of the former LFIA systems and to offer other prospective functionalities for LFIA quantification.
尽管定量侧向流动免疫分析（LFIA）已经广泛渗透到各个市场，但在某些情况下，它仍存在灵敏度问题。为了解决这个问题，本研究提出了一种优化的 LFIA 定量比色读出方法。开发了一种利用彩色相机的检测读取设备和一种使用 Bayer 滤波图像的分析方法。对检测线进行光谱测量，以确定包含检测线信息并有效最小化噪声的颜色通道。在绿色通道中，随着样品中目标物质浓度的增加，强度比的变化最大。输出曲线的线性范围为 0 至 10ng/mL，检测限为 2ng/mL。所提出的仪器和分析方法有望有效解决先前 LFIA 系统的低灵敏度问题，并为 LFIA 定量提供其他有前景的功能。
