Gussenhoven G C, van der Hoorn M A, Goris M G, Terpstra W J, Hartskeerl R A, Mol B W, van Ingen C W, Smits H L
Department of Biomedical Research, Royal Tropical Institute, AZ Amsterdam, The Netherlands.
J Clin Microbiol. 1997 Jan;35(1):92-7. doi: 10.1128/jcm.35.1.92-97.1997.
We studied a dipstick assay for the detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human serum samples. A high degree of concordance was observed between the results of the dipstick assay and an IgM enzyme-linked immunosorbent assay (ELISA). Application of the dipstick assay for the detection of acute leptospirosis enabled the accurate identification, early in the disease, of a high proportion of the cases of leptospirosis. Analysis of a second serum sample is recommended, in order to determine seroconversion or increased staining intensity. All serum samples from the patients who were confirmed to be positive for leptospirosis by either a positive microscopic agglutination test or a positive culture but were found to be negative by the dipstick assay were also judged to be negative by the IgM ELISA or revealed borderline titers by the IgM ELISA. Some cross-reactivity was observed for sera from patients with diseases other than leptospirosis, and this should be taken into account in the interpretation of test results. The dipstick assay is easy to perform, can be performed quickly, and requires no electricity or special equipment, and the assay components, a dipstick and a staining reagent, can be stored for a prolonged period without a loss of reactivity, even at elevated temperatures.
我们研究了一种用于检测人血清样本中钩端螺旋体特异性免疫球蛋白M（IgM）抗体的试纸条检测法。试纸条检测法与IgM酶联免疫吸附测定（ELISA）的结果之间观察到高度一致性。应用试纸条检测法检测急性钩端螺旋体病能够在疾病早期准确识别出高比例的钩端螺旋体病病例。建议分析第二份血清样本，以确定血清转化或染色强度增加。通过显微镜凝集试验阳性或培养阳性确诊为钩端螺旋体病阳性但试纸条检测法结果为阴性的患者的所有血清样本，经IgM ELISA检测也判定为阴性或IgM ELISA显示临界滴度。观察到非钩端螺旋体病患者的血清存在一些交叉反应，在解释检测结果时应考虑到这一点。试纸条检测法易于操作，检测速度快，无需电力或特殊设备，并且检测组件（试纸条和染色试剂）即使在高温下也可长期保存而不丧失反应性。
