Liu Sijie, Dou Leina, Yao Xiaolin, Zhang Wentao, Zhao Man, Yin Xuechi, Sun Jing, Zhang Daohong, Wang Jianlong
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, And Beijing Laboratory for Food Quality and Safety, Beijing, 100193, China.
Biosens Bioelectron. 2020 Dec 1;169:112610. doi: 10.1016/j.bios.2020.112610. Epub 2020 Sep 15.
Development of sensitive, facile and rapid biosensors is important for widespread applications. Nanozymes can be ideal signal donors for constructing dual-readout lateral flow immunoassays (LFIA) because they are an excellent class of optical reporters. Herein, a magnetic prussian blue nanozyme (MPBN) mediated dual-readout on-demand multiplex lateral flow immunoassay (MLFIA) was established by employing ractopamine (RAC) and clenbuterol (CLE) as the model analytes. The MPBN was synthesized through in-suit shell-growing and introduced as a bifunctional signal tag owing to their darker original color and peroxidase-like activity. Based on the catalytic signal created by catalyzing oxidation of chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) and colorimetric signal generated by tag's original color, improved precision and broadened detection range were acquired by implementing a dual-readout strategy. And a two-fold increase in the detection range could fulfill different limit requirements of the same target in various regions. The obtained recoveries from 84.01% to 119.94% indicating the repeatability and reliability of the proposed method. This method provides an attractive platform for the detection of a same target with different detection limits, which possesses a considerable potential in monitoring of other targets.
开发灵敏、简便且快速的生物传感器对于广泛应用而言至关重要。纳米酶可成为构建双读出侧向流动免疫分析（LFIA）的理想信号供体，因为它们是一类出色的光学报告分子。在此，通过采用莱克多巴胺（RAC）和克伦特罗（CLE）作为模型分析物，建立了一种磁性普鲁士蓝纳米酶（MPBN）介导的按需双读出多重侧向流动免疫分析（MLFIA）。MPBN通过原位壳生长合成，并因其较深的原色和类过氧化物酶活性而被引入作为双功能信号标签。基于催化显色底物3,3',5,5'-四甲基联苯胺（TMB）氧化产生的催化信号以及标签原色产生的比色信号，通过实施双读出策略获得了更高的精度和更宽的检测范围。检测范围扩大两倍可满足同一目标在不同地区的不同限量要求。所获得的回收率在84.01%至119.94%之间，表明该方法具有可重复性和可靠性。该方法为检测具有不同检测限的同一目标提供了一个有吸引力的平台，在监测其他目标方面具有相当大的潜力。
