Laboratoire de Transformations Intégrées de la Matière Renouvelable, EA 4297, Centre de Recherches de Royallieu, Sorbonne Universités, Université de Technologie de Compiègne, BP 20529, 60205, Compiègne Cedex, France.
Institute of Biocolloidal Chemistry named after F. D. Ovcharenko, NAS of Ukraine, 42, blvr. Vernadskogo, Kyiv, 03142, Ukraine.
Bioprocess Biosyst Eng. 2019 Feb;42(2):173-186. doi: 10.1007/s00449-018-2038-5. Epub 2018 Nov 23.
Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the prevention and treatment of human diseases. Moreover, some species of Nannochloropsis are the promising sources of biofuels and excellent candidates for the replacement of classical biofuel crops. This review describes and compares the efficiency of different conventional and novel techniques that can be used for cell disruption and recovery of bio-molecules from Nannochloropsis sp. Classification of different extraction techniques includes chemical, enzymatic, mechanical and other physical methods. The detailed analysis of extraction efficiency assisted by pressure and temperature (subcritical and supercritical fluids, hydrothermal liquefaction), ultrasound, microwaves, and pulsed electric energy (pulsed electric fields and high voltage electrical discharges) is presented. The general discussion includes comparison between techniques, their effectiveness for cell disruption and selectivity of bio-molecules extraction from Nannochloropsis sp. The cost-effectiveness, benefits and limitations of different techniques are also analyzed.
微藻中的盐生杜氏藻属是有价值的生物分子（如色素、脂类、蛋白质）来源，具有预防和治疗人类疾病的潜力。此外，一些盐生杜氏藻属的物种是生物燃料的有前途的来源，也是替代传统生物燃料作物的优秀候选者。本文描述并比较了不同常规和新型技术从盐生杜氏藻属中细胞破碎和回收生物分子的效率。不同提取技术的分类包括化学、酶、机械和其他物理方法。详细分析了压力和温度（亚临界和超临界流体、水热液化）、超声、微波和脉冲电能（脉冲电场和高压电放电）辅助下的提取效率。一般性讨论包括技术比较、细胞破碎的有效性以及从盐生杜氏藻属中选择性提取生物分子。还分析了不同技术的成本效益、优点和局限性。
