DVGW-Technologiezentrum Wasser (TZW), Department of Microbiology and Molecular Biology, Karlsruher Straße 84, Karlsruhe, Germany.
Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Sipping Road, Shanghai, People's Republic of China.
Sci Total Environ. 2019 Mar 10;655:337-346. doi: 10.1016/j.scitotenv.2018.11.211. Epub 2018 Nov 16.
Lake Tai is China's third largest freshwater lake and an important water resource for agriculture, industrial sectors, and as drinking water for several large cities. In this study, the occurrence of five antibiotic resistance genes (sul1, bla, bla, bla, mcr-1) was investigated in water and sediment samples collected from Lake Tai. Antibiotic resistances are currently increasing, posing a significant threat to public health. The sulfonamide resistance gene sul1 was highly abundant in all analyzed water and sediment samples. In addition, the two β-lactamase genes blaand bla - encoding clinically relevant antibiotic resistances - were detected in 67.1 and 7.3% of the water samples and in 70.7 and 15.4% of the sediment samples. The third β-lactamase gene, bla, was only detected in water samples (13.4%), while the colistin resistance gene mcr-1 was not detected in any of the samples. No significant variations between different sampling sites or time points could be observed. The investigation of drinking water treatment at Lake Tai, using lake water as influent, showed a significant reduction of the antibiotic resistance genes through the treatment process. Microbial source tracking showed only low fecal contamination by humans, ruminants, and pigs, indicating the relevance of other sources such as fish farms. Overall, our results provide important insights into the occurrence and abundance of antibiotic resistance genes in the Lake Tai water system and their elimination via drinking water treatment.
太湖是中国第三大淡水湖，是农业、工业部门的重要水资源，也是几个大城市的饮用水源。本研究调查了取自太湖的水样和底泥样品中 5 种抗生素耐药基因（sul1、blaTEM、blaCTX-M、blaOXA 和 mcr-1）的出现情况。抗生素耐药性目前正在增加，对公众健康构成重大威胁。磺胺类耐药基因 sul1 在所有分析的水样和底泥样品中都高度丰富。此外，编码临床相关抗生素耐药性的两种β-内酰胺酶基因 blaTEM 和 blaCTX-M 在 67.1%和 7.3%的水样以及 70.7%和 15.4%的底泥样品中被检出。第三种β-内酰胺酶基因 bla 仅在水样中检出（13.4%），而黏菌素耐药基因 mcr-1 未在任何样品中检出。未观察到不同采样点或时间点之间存在显著差异。对以太湖水作为进水的太湖饮用水处理的调查显示，通过处理过程显著降低了抗生素耐药基因的数量。微生物源追踪显示，只有人类、反刍动物和猪的粪便污染程度较低，表明其他来源（如养殖场）的相关性。总的来说，我们的研究结果为太湖水系中抗生素耐药基因的出现和丰度及其通过饮用水处理消除提供了重要的见解。
