Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Gene. 2019 Mar 1;687:298-307. doi: 10.1016/j.gene.2018.11.053. Epub 2018 Nov 22.
Dof proteins comprise a plant-specific transcription factor family involved in regulation of many plant processes. Despite their diverse roles, little information is available in cassava (Manihot esculenta, Euphorbiaceae), the sixth major staple crop in the world. This study presents the first genome-wide identification of Dof family genes in cassava, resulting in 45 family members that are unevenly distributed across 17 out of the 18 chromosomes. Phylogenetic analysis assigned these genes into eight groups representing four subfamilies. Synteny analysis and BRH (Best Reciprocal Hit) method were also adopted to study evolutionary relationships. Among 21 MeDof duplicates identified, the majority of them were derived from the recent whole-genome duplication (WGD), whereas only one from local duplication. Transcriptional profiling revealed diverse expression patterns among various tissues, and further comparison of conserved motifs and expression profiles revealed divergence of MeDof duplicates. These findings provide valuable information for functional analysis of Dof genes in cassava.
Dof 蛋白是一类参与调控多种植物生理过程的植物特异性转录因子家族。尽管它们具有多样化的功能，但在世界第六大主要主食作物木薯（Manihot esculenta，大戟科）中，有关它们的信息却很少。本研究首次对木薯中的 Dof 家族基因进行了全基因组鉴定，结果在 18 条染色体中的 17 条染色体上发现了 45 个家族成员，它们的分布不均匀。系统发育分析将这些基因分为代表四个亚家族的八个组。共鉴定出 21 个 MeDof 基因的重复序列，它们大多来自最近的全基因组复制（WGD），而仅有一个来自局部复制。转录谱分析显示了不同组织之间的多样化表达模式，进一步比较保守基序和表达谱揭示了 MeDof 基因重复序列的分化。这些发现为木薯 Dof 基因的功能分析提供了有价值的信息。
