Cai Kewei, Xie Xiaoyu, Han Lu, Chen Junbo, Zhang Jinwang, Yuan Hongtao, Shen Jiajia, Ren Yishuang, Zhao Xiyang
Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Front Plant Sci. 2024 May 8;15:1412175. doi: 10.3389/fpls.2024.1412175. eCollection 2024.
Populus simonii, a notable native tree species in northern China, demonstrates impressive resistance to stress, broad adaptability, and exceptional hybridization potential. DOF family is a class of specific transcription factors that only exist in plants, widely participating in plant growth and development, and also playing an important role in abiotic stress response. To date, there have been no reported studies on the DOF gene family in P. simonii, and the expression levels of this gene family in different tissues of poplar, as well as its expression patterns under cold, heat, and other stress conditions, remain unclear.
In this study, DOF gene family were identified from the P. simonii genome, and various bioinformatics data on the DOF gene family, gene structure, gene distribution, promoters and regulatory networks were analyzed. Quantitative real time PCR technology was used to verify the expression patterns of the DOF gene family in different poplar tissues.
This research initially pinpointed 41 PSDOF genes in P. simonii genome. The chromosomal localization results revealed that the PSDOF genes is unevenly distributed among 19 chromosomes, with the highest number of genes located on chromosomes 4, 5, and 11. A phylogenetic tree was constructed based on the homology between Arabidopsis thaliana and P. simonii, dividing the 41 PSDOF genes into seven subgroups. The expression patterns of PSDOF genes indicated that specific genes are consistently upregulated in various tissues and under different stress conditions, suggesting their pivotal involvement in both plant development and response to stress. Notably, PSDOF35 and PSDOF28 serve as pivotal hubs in the interaction network, playing a unique role in coordinating with other genes within the family.
The analysis enhances our comprehension of the functions of the DOF gene family in tissue development and stress responses within P. simonii. These findings provide a foundation for future exploration into the biological roles of DOF gene family.
小叶杨是中国北方一种著名的本土树种，具有显著的抗逆性、广泛的适应性和出色的杂交潜力。DOF家族是一类仅存在于植物中的特定转录因子，广泛参与植物的生长发育，在非生物胁迫响应中也发挥着重要作用。迄今为止，尚未有关于小叶杨DOF基因家族的报道，该基因家族在杨树不同组织中的表达水平以及在冷、热等胁迫条件下的表达模式仍不清楚。
本研究从小叶杨基因组中鉴定出DOF基因家族，并分析了DOF基因家族的各种生物信息学数据、基因结构、基因分布、启动子和调控网络。采用实时定量PCR技术验证DOF基因家族在不同杨树组织中的表达模式。
本研究首次在小叶杨基因组中确定了41个PSDOF基因。染色体定位结果显示，PSDOF基因在19条染色体上分布不均，其中4号、5号和11号染色体上的基因数量最多。基于拟南芥和小叶杨之间的同源性构建了系统发育树，将41个PSDOF基因分为七个亚组。PSDOF基因的表达模式表明，特定基因在各种组织和不同胁迫条件下持续上调，表明它们在植物发育和胁迫响应中都起着关键作用。值得注意的是，PSDOF35和PSDOF28是相互作用网络中的关键枢纽，在与家族内其他基因的协调中发挥着独特作用。
该分析增强了我们对DOF基因家族在小叶杨组织发育和胁迫响应中功能的理解。这些发现为未来探索DOF基因家族的生物学作用提供了基础。
