Department of Physics, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Biosens Bioelectron. 2019 Feb 1;126:478-484. doi: 10.1016/j.bios.2018.11.025. Epub 2018 Nov 16.
The in-situ and rapid detection of live and dead bacteria is essential for human and environmental care. It has become one of the biggest needs in the biological and medical sciences to prevent infectious diseases, which usually occur in hospitals and field clinics. In the current scenario, antibiotic resistance is one of the severe public health problems, which requires a quick and efficient solution. Here, we report a facile sensitive, portable, user-friendly, cost-effective and time saving approach for detection of live, dead and drug-resistant bacteria. The endogenous HS evolution was targeted to differentiate between live and dead as well as antibiotic resistant bacteria. The silver nanorods (AgNRs) arrays sensors were fabricated by glancing angle deposition technique. The colorimetric and water wettability features of as-synthesized AgNRs are found to be highly sensitive and selective for HS. E. coli. P. aeruginosa, B. subtilis and S. aureus were used as a model organism in this study. All the bacteria were found to produce HS by their metabolism process. In order to detect the antibiotic resistant E. coli were grown in the presence of different concentration of ampicillin in Luria broth. A drastic visible change in color as well as wetting of AgNRs array was observed. To make the technique easy, a user-friendly and field deployable mobile app 'Colorimetric Detector' was developed. This technique takes only 4-6 h whereas the conventional methods need around 24 h for the same. This dual mode facile and, inexpensive method can be easily scaled up in the field of diagnostics.
原位和快速检测活细菌和死细菌对于人类和环境的保护至关重要。防止传染病在医院和现场诊所发生，已成为生物和医学科学的最大需求之一。在当前情况下，抗生素耐药性是严重的公共卫生问题之一，需要快速有效的解决方案。在这里，我们报告了一种简便、灵敏、便携、用户友好、经济高效且节省时间的方法，用于检测活细菌、死细菌和耐药细菌。内源性 HS 进化被靶向用于区分活细菌和死细菌以及耐药细菌。通过掠角沉积技术制造了银纳米棒 (AgNRs) 阵列传感器。所合成的 AgNRs 的比色和润湿性特征被发现对 HS 具有高度的灵敏性和选择性。大肠杆菌、铜绿假单胞菌、枯草芽孢杆菌和金黄色葡萄球菌被用作本研究的模型生物。所有细菌都被发现通过代谢过程产生 HS。为了检测耐药大肠杆菌，在含有不同浓度氨苄青霉素的 LB 肉汤中培养。观察到 AgNRs 阵列的颜色和润湿性发生了明显的变化。为了使技术变得简单，开发了一个用户友好且可在现场部署的移动应用程序“比色探测器”。该技术仅需 4-6 小时，而传统方法则需要大约 24 小时。这种双模式简便且经济的方法可以很容易地在诊断领域中推广。
