Guangdong Provincial Engineering Research Center of Public Health Detection and Assessment, Guangdong Pharmaceutical University, Guangzhou, 510310, China.
Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Mikrochim Acta. 2021 Sep 6;188(10):322. doi: 10.1007/s00604-021-04981-0.
A rapid method for colorimetric monitoring of bacterial viability is described. The colorimetric method was carried out based on glucose oxidase-encapsulated Zn/Co-infinite coordination polymer (Zn/Co-ICP@GOx), which was prepared in aqueous solution free of toxic organic solvents at room temperature. The Zn/Co-ICP@GOx was confirmed to be a robust sphere structure with an average diameter of 147.53 ± 20.40 nm. It integrated the catalytic activity of natural enzyme (GOx) and mimetic peroxidase (Co (П)) all in one, efficiently acting as a biocatalytic cascade platform for glucose catalytic reaction. Exhibiting good multi-enzyme catalytic activity, stability, and selectivity, Zn/Co-ICP@GOx can be used for colorimetric glucose detection. The linear range was 0.01-1.0 mmol/L, and the limit of detection (LOD) was 0.005 mmol/L. As the glucose metabolism is a common expression of bacteria, the remaining glucose can indirectly represent the bacterial viability. Hence, a Zn/Co-ICP@GOx-based colorimetric method was developed for monitoring of bacterial viability. The color was intuitively observed with the naked eye, and the bacterial viability was accurately quantified by measurement of the absorbance at 510 nm. The method was applied to determination of bacterial viability in water and milk samples with recoveries of 99.0-103% and RSD of 0.43-7.5%. The method was rapid (less than 40 min) and applicable to different bacterial species irrespective of Gram-positive and Gram-negative bacteria, providing a universal and promising strategy for real-time monitoring of bacterial viability.
一种用于比色监测细菌活力的快速方法被描述。比色法是基于葡萄糖氧化酶封装的 Zn/Co-无限配位聚合物（Zn/Co-ICP@GOx）进行的，该聚合物是在室温下于不含有毒有机溶剂的水溶液中制备的。Zn/Co-ICP@GOx 被证实为具有 147.53 ± 20.40nm 平均直径的坚固球体结构。它将天然酶（GOx）的催化活性和模拟过氧化物酶（Co(П)）的催化活性集成在一起，作为葡萄糖催化反应的高效生物催化级联平台。Zn/Co-ICP@GOx 表现出良好的多酶催化活性、稳定性和选择性，可用于比色葡萄糖检测。线性范围为 0.01-1.0mmol/L，检测限（LOD）为 0.005mmol/L。由于葡萄糖代谢是细菌的常见表现，剩余的葡萄糖可以间接代表细菌的活力。因此，开发了一种基于 Zn/Co-ICP@GOx 的比色法来监测细菌活力。可以通过肉眼直观地观察颜色，并通过测量 510nm 处的吸光度来准确地定量细菌活力。该方法已应用于水和牛奶样品中细菌活力的测定，回收率为 99.0-103%，相对标准偏差（RSD）为 0.43-7.5%。该方法快速（少于 40 分钟），适用于不同的细菌种类，无论革兰氏阳性菌还是革兰氏阴性菌，为实时监测细菌活力提供了一种通用且有前景的策略。
