Guy K, Crichton D N, Ross J A
MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, U.K.
J Immunol Methods. 1988 Sep 13;112(2):261-5. doi: 10.1016/0022-1759(88)90366-3.
We describe a method of immunofluorescence which is a lateral application of the principles of the APAAP immunohistochemical technique. Immune complexes of R-phycoerythrin and monoclonal anti-R-phycoerythrin (PEAPE complexes) were used in an indirect immunofluorescence technique to detect the binding to cells of monoclonal antibodies directed to IgM, HLA-DR and B cell activation and differentiation antigens. PEAPE complexes were linked to cell surface bound mAbs by unlabelled anti-mouse Ig antibodies to produce high levels of fluorescent staining. The sensitivity of this method of indirect immunofluorescence was enhanced by the sequential application of several cycles of anti-mouse Ig and PEAPE complexes.
