Lyu Fengjiao, Blauch Lucas R, Tang Sindy K Y
Department of Mechanical Engineering, Stanford University, Stanford, CA, United States.
Department of Mechanical Engineering, Stanford University, Stanford, CA, United States.
Methods Cell Biol. 2018;148:133-159. doi: 10.1016/bs.mcb.2018.09.006. Epub 2018 Oct 25.
This book chapter describes the use of droplet microfluidics to phenotype single cells. The basic process flow includes the encapsulation of single cells with a specific probe into aqueous micro-droplets suspended in a biocompatible oil. The probe is chosen to measure the phenotype of interest. After incubation, the encapsulated cell turns the probe fluorescent and renders the entire droplet fluorescent. Enumerating drops that are fluorescent quantifies the concentration of cells possessing the phenotype of interest. Examining the distribution of fluorescence further allows one to quantify the heterogeneity among the cell population.
本章介绍了利用微滴微流控技术对单细胞进行表型分析的方法。基本流程包括将单个细胞与特定探针封装到悬浮在生物相容性油中的水性微滴中。所选探针用于测量感兴趣的表型。孵育后，被封装的细胞使探针发出荧光，进而使整个微滴发出荧光。对发出荧光的微滴进行计数可量化具有感兴趣表型的细胞浓度。进一步检查荧光分布可使人们量化细胞群体中的异质性。
