College of Animal Science and Technology, Southwest University, Chongqing 400715, China.
College of Animal Science and Technology, Southwest University, Chongqing 400715, China; Research Center of Fishery Resource and Environment, Southwest University, Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Chongqing 400715, China.
J Microbiol Immunol Infect. 2020 Aug;53(4):550-560. doi: 10.1016/j.jmii.2018.10.006. Epub 2018 Nov 9.
Mast cells play a central role in innate and adaptive immunity by releasing pre-formed and de novo synthesized mediators, which include microRNAs. Although miRNAs have been confirmed to function in cell proliferation, differentiation, apoptosis, and the immune response, their functions are still limited in mast cells degranulation.
Here, we survey miRNA expression profiles in activated mouse bone marrow-derived mast cells (BMMCs) with a miRNA microarray and compare the profiles to those from resting BMMCs. Partial miRNAs were selected for confirmation by qPCR, and let-7i was selected for function discover in mast cell degranulation process. TargetScan Mouse database were used for target genes prediction, gene ontology (GO) were used for gene molecular function classifications, and Cytoscape software were used to construct gene network of degranulation.
We found 13 up-regulated miRNAs and 7 down-regulated miRNAs in DNP activated BMMCs by miRNA microarray; and let-7b, let-7c, let-7d, let-7f, let-7i, and miR-652 were up-regulated, and miR-296-3p was down-regulated in DNP-stimulated BMMCs by qPCR. In the function research, let-7i can inhibit mast cell degranulation by suppress Exco8 expression. Overall, the data indicate that miRNAs participate in mast cell activation, especial for mast cell degranulation process.
肥大细胞通过释放预先形成和新合成的介质，包括 microRNAs，在先天和适应性免疫中发挥核心作用。虽然 microRNAs 已被证实可在细胞增殖、分化、凋亡和免疫反应中发挥作用，但它们在肥大细胞脱颗粒中的功能仍然有限。
在这里，我们使用 microRNA 微阵列调查激活的小鼠骨髓来源的肥大细胞（BMMC）中的 microRNA 表达谱，并将其与静息 BMMC 的谱进行比较。部分 microRNAs 通过 qPCR 进行确认选择，let-7i 被选择用于在肥大细胞脱颗粒过程中发现功能。TargetScan Mouse 数据库用于预测靶基因，基因本体论（GO）用于基因分子功能分类，Cytoscape 软件用于构建脱颗粒的基因网络。
我们通过 microRNA 微阵列发现 DNP 激活的 BMMC 中有 13 个上调的 microRNAs 和 7 个下调的 microRNAs；通过 qPCR 发现 DNP 刺激的 BMMC 中 let-7b、let-7c、let-7d、let-7f、let-7i 和 miR-652 上调，miR-296-3p 下调。在功能研究中，let-7i 可以通过抑制 Exco8 的表达抑制肥大细胞脱颗粒。总的来说，数据表明 microRNAs 参与肥大细胞的激活，特别是肥大细胞脱颗粒过程。
