Curtis R, Cohen J, Fok-Seang J, Hanley M R, Gregson N A, Reynolds R, Wilkin G P
Biochemistry Department, Imperial College of Science and Technology, London, UK.
J Neurocytol. 1988 Feb;17(1):43-54. doi: 10.1007/BF01735376.
The origin of oligodendrocytes and astrocytes in the CNS is still a focus of much experimentation and controversy. We have used antibodies against ganglioside GD3 and galactocerebroside (GC) to follow the origin and development of rat cerebellar oligodendrocytes both in vitro and in vivo. The immunofluorescent identification of GC+ cells in the rat neonatal cerebellum in vivo, revealed that cells initially GD3+/GC- appeared to make the transition via GD3+/GC+ cells to GD3-/GC+ oligodendrocytes. This sequence of events closely paralleled the maturation of cerebellar oligodendrocyte precursors found in serum-free dissociated culture. In contrast, whereas both GD3+ and glial fibrillary acidic protein-positive cells were seen in serum-containing dissociated culture and also in freshly dissociated suspensions of cerebellum at postnatal days 0 to 6, such cells could not be identified in situ. Putative GD3+/GC- oligodendrocyte precursor cells arose from the deeper regions of the cerebellum at birth, perhaps initially from the superior medullary velum adjacent to the fourth ventricle, and appeared to migrate into the developing folia just prior to myelination and the acquisition of GC.
中枢神经系统中少突胶质细胞和星形胶质细胞的起源仍是大量实验和争议的焦点。我们使用了抗神经节苷脂GD3和半乳糖脑苷脂（GC）的抗体，在体外和体内追踪大鼠小脑少突胶质细胞的起源和发育。对体内大鼠新生小脑GC+细胞的免疫荧光鉴定显示，最初GD3+/GC-的细胞似乎通过GD3+/GC+细胞转变为GD3-/GC+少突胶质细胞。这一系列事件与无血清解离培养中发现的小脑少突胶质细胞前体的成熟过程密切平行。相比之下，虽然在含血清的解离培养物以及出生后0至6天的新鲜解离小脑悬液中都能看到GD3+和胶质纤维酸性蛋白阳性细胞，但在原位无法识别此类细胞。假定的GD3+/GC-少突胶质细胞前体细胞在出生时起源于小脑的较深区域，可能最初来自与第四脑室相邻的上髓帆，并且似乎在髓鞘形成和获得GC之前迁移到发育中的小叶。
