Instituto de Ciencias Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
Escuela de Medicina Veterinaria, Facultad de Ciencias, Universidad Mayor Sede Temuco, Chile.
Ticks Tick Borne Dis. 2019 Feb;10(2):321-327. doi: 10.1016/j.ttbdis.2018.11.009. Epub 2018 Nov 17.
Canine piroplasmoses, caused by Babesia spp., Theileria spp. and Rangelia vitalii, are emerging vector-borne diseases with a worldwide distribution, transmitted by ticks. The aim of this study was to determine the prevalence and perform molecular characterization of piroplasmids in domestic dogs from Asunción city, Paraguay. Blood samples were taken from 384 domestic dogs from Asunción city, Paraguay. DNA was purified from dog blood samples and submitted to nested PCR assays for piroplasmids (18S rRNA) and sequenced for identification and phylogenetic analysis. Overall piroplasmid prevalence in dogs from Paraguay was 6% (23/384 [CI 95% = 3.6-8.4%]). Phylogenetic studies showed that Babesia vogeli was the most prevalent species (91% [21/23]), followed by Theileria equi (4% [1/23]) and Rangelia sp. closely related to R. vitalii (4% [1/23]). Babesia vogeli, T. equi and Rangelia sp. circulate among domestic dogs from Asunción city, and are described for the first time in Paraguay.
犬巴贝斯虫病、泰勒虫病和兰格尔热，由巴贝斯虫属、泰勒虫属和兰格尔热属引起，是一种具有全球分布的新兴虫媒传染病，通过蜱传播。本研究旨在确定巴拉圭亚松森市的家养犬中巴贝斯虫病的流行率并进行分子特征分析。从巴拉圭亚松森市的 384 只家养犬中采集血液样本。从犬血样中提取 DNA，进行针对巴贝斯虫（18S rRNA）的巢式 PCR 检测，并对鉴定和系统发育分析进行测序。来自巴拉圭的犬的总体巴贝斯虫病流行率为 6%（23/384 [95%CI 3.6-8.4%]）。系统发育研究表明，巴贝斯虫 vogeli 是最常见的物种（91%[21/23]），其次是马泰勒虫（4%[1/23]）和与兰格尔热属密切相关的兰格尔热属（4%[1/23]）。巴贝斯虫 vogeli、马泰勒虫和兰格尔热属在亚松森市的家养犬中传播，这是它们在巴拉圭的首次描述。
